National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, P. R. China.
Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China.
Biomacromolecules. 2022 Apr 11;23(4):1680-1692. doi: 10.1021/acs.biomac.1c01616. Epub 2022 Mar 8.
Programing self-assembly of naturally bioactive molecules has been a wide topic of great significance for biomedical uses. Despite the fact that plant-derived polyphenols with catechol or pyrogallol moieties have been widely studied to construct nanocomplexes or nanocoatings via self-polymerization, there is no report on the self-assembly of these polyphenols into therapeutic hydrogels for potential applications. Here, we reported that adding a very small amount of resveratrol (Res) into the gallic acid (GA) aqueous solution could trigger the quick self-assembly of GA to form a fibrous hydrogel within 5 min through hydrogen bonds and π-π interactions. The length of GA/Res (GR) fibrils in gels varied from 100 to 1000 microns, with a diameter of around 1 μm. Notably, these GR hydrogels showed excellent colloid stability, providing better slow release and outstanding biocompatibility. Also, experiments indicated the hydrogels had high antibacterial effects and excellent wound healing capabilities in a total skin defect model via regulating the expression of inflammatory factors (-6, -1β, and -α) due to the release of therapeutic agents (GA and Res) into the matrix. Overall, our results provide a new strategy to accelerate self-assembly of GA by adding Res to form hydrogels, which is further proved as a promising therapeutic carrier for wound healing.
将天然生物活性分子进行编程自组装对于生物医学应用具有重要意义。尽管具有邻苯二酚或焦儿茶酚部分的植物衍生多酚已被广泛研究用于通过自聚合来构建纳米复合物或纳米涂层，但尚未有关于这些多酚自组装成治疗性水凝胶以用于潜在应用的报道。在这里，我们报道了在没食子酸（GA）水溶液中加入极少量白藜芦醇（Res）可以通过氢键和π-π相互作用在 5 分钟内快速引发 GA 的自组装，形成纤维状水凝胶。凝胶中 GA/Res（GR）纤维的长度从 100 到 1000 微米不等，直径约为 1 微米。值得注意的是，这些 GR 水凝胶表现出优异的胶体稳定性，提供了更好的缓慢释放和出色的生物相容性。此外，实验表明，由于治疗剂（GA 和 Res）释放到基质中，水凝胶通过调节炎症因子（-6、-1β 和 -α）的表达，在全层皮肤缺损模型中具有高抗菌作用和出色的伤口愈合能力。总的来说，我们的研究结果提供了一种通过添加 Res 来加速 GA 自组装形成水凝胶的新策略，进一步证明其作为一种有前途的伤口愈合治疗载体。
