Departments of Polymer Science and Engineering & Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 34134, Republic of Korea.
School of Textile and Clothing, Nantong University, Nantong 226019, China; Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
Carbohydr Polym. 2021 May 15;260:117808. doi: 10.1016/j.carbpol.2021.117808. Epub 2021 Feb 17.
In the present study, a novel synthetic tissue adhesive material capable of sealing wounds without the use of any crosslinking agent was developed by conjugating thermosensitive hexanoyl glycol chitosan (HGC) with gallic acid (GA). The degree of N-gallylation was manipulated to prepare GA-HGCs with different GA contents. GA-HGCs demonstrated thermosensitive sol-gel transition behavior and formed irreversible hydrogels upon natural oxidation of the pyrogallol moieties in GA, possibly leading to GA-HGC crosslinks through intra/intermolecular hydrogen bonding and chemical bonds. The GA-HGC hydrogels exhibited self-healing properties, high compressive strength, strong tissue adhesive strength and biodegradability that were adjustable according to the GA content. GA-HGCs also presented excellent biocompatibility and wound healing effects. The results of in vivo wound healing efficacy studies on GA-HGC hydrogels indicated that they significantly promote wound closure and tissue regeneration by upregulating growth factors and recruiting fibroblasts compared to the untreated control group.
在本研究中，通过将热敏性己酰化壳聚糖（HGC）与没食子酸（GA）偶联，开发出了一种新型的无需使用任何交联剂即可密封伤口的合成组织粘合剂材料。通过控制 N-酰化程度，制备了具有不同 GA 含量的 GA-HGC。GA-HGC 表现出热敏溶胶-凝胶转变行为，并在 GA 中的焦没食子酚部分自然氧化的作用下形成不可逆的水凝胶，可能通过分子内/分子间氢键和化学键导致 GA-HGC 交联。GA-HGC 水凝胶具有自修复性能、高抗压强度、强组织黏附强度和可根据 GA 含量进行调节的生物降解性。GA-HGC 还表现出良好的生物相容性和伤口愈合效果。GA-HGC 水凝胶体内伤口愈合功效研究的结果表明，与未处理的对照组相比，它们通过上调生长因子和募集成纤维细胞，显著促进伤口闭合和组织再生。
