绵羊和小鼠透明带对顶体蛋白酶的形态学和分子易感性

The morphological and molecular susceptibility of sheep and mouse zona pellucida to acrosin.

作者信息

Brown C R

出版信息

J Reprod Fertil. 1986 Jul;77(2):411-7. doi: 10.1530/jrf.0.0770411.

DOI:10.1530/jrf.0.0770411

Abstract

The effect of acrosin on the gross morphology and macromolecular constituents of the mouse and sheep zona pellucida has been examined by light microscopy and SDS-PAGE following labelling of the zona with 125I. Ram and boar acrosin had similar effects on the sheep zona in that while there was no discernible alteration in the gross morphology of the investment it was proteolysed to the same limited extent by both enzymes; during 1 h major polypeptides of Mr 180,000 (present on the zona pellucida of eggs but absent from that of oocytes) and 80,000 were hydrolysed, giving rise to polypeptides of Mr 68,000, 54,000 and 46,000, of which the last accumulated and represented the lowest molecular weight hydrolysis product. By contrast, the mouse zona pellucida was completely dissolved from the egg by ram acrosin and the investment's macromolecules were extensively hydrolysed.

摘要

在用¹²⁵I标记透明带后，通过光学显微镜和SDS-PAGE研究了顶体蛋白酶对小鼠和绵羊透明带的大体形态和大分子成分的影响。公羊和公猪的顶体蛋白酶对绵羊透明带具有相似的作用，即虽然该覆盖物的大体形态没有明显改变，但两种酶对其进行了相同程度的有限蛋白水解；在1小时内，分子量为180,000（存在于卵的透明带上，但卵母细胞的透明带上不存在）和80,000的主要多肽被水解，产生分子量为68,000、54,000和46,000的多肽，其中最后一种多肽积累并代表最低分子量的水解产物。相比之下，公羊顶体蛋白酶可使小鼠透明带从卵上完全溶解，并且该覆盖物的大分子被广泛水解。