State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China.
Phytochemistry. 2022 Jun;198:113155. doi: 10.1016/j.phytochem.2022.113155. Epub 2022 Mar 5.
Cynomorium coccineum subsp. songaricum (Rupr.) J. Leonard has been widely used as a Chinese herbal remedy or a functional food for treating symptoms of aging or neurodegenerative diseases. A further investigation on the finding of active constituents led to the isolation and identification of four previously undescribed triterpenoids, together with 20 known compounds. Their structures were elucidated by extensive spectroscopic analysis (IR, NMR, HRMS, and CD). Sixteen compounds showed significant neuroprotective effects against glutamate-induced or oxygen-glucose deprivation-induced SK-N-SH cell death. Our findings revealed the active constituents of C. coccineum subsp. songaricum and indicated that both oleanane-type and ursane-type triterpenes could be valuable platforms for neurodegenerative agents based on primary structure-activity relationship analysis.
锁阳（Cynomorium coccineum subsp. songaricum (Rupr.) J. Leonard）作为一种中草药或功能性食品，被广泛用于治疗衰老或神经退行性疾病的症状。对活性成分的进一步研究导致了四种以前未描述的三萜类化合物的分离和鉴定，以及 20 种已知化合物。通过广泛的光谱分析（IR、NMR、HRMS 和 CD）阐明了它们的结构。十六种化合物对谷氨酸诱导或氧葡萄糖剥夺诱导的 SK-N-SH 细胞死亡显示出显著的神经保护作用。我们的研究结果揭示了锁阳亚种的活性成分，并表明齐墩果烷型和熊果烷型三萜类化合物都可以作为神经退行性药物的有价值的平台，这基于初步的结构-活性关系分析。
