Battifora H
Lab Invest. 1986 Aug;55(2):244-8.
We describe a method of embedding 100 or more different tissue samples in a normal-sized paraffin block, the multitumor tissue block (MTTB). MTTBs allow the simultaneous immunohistologic testing of numerous tissue samples on a single slide with one drop of antibody. Because all of the tissue samples are treated equally during immunostaining, most sources of variation are removed and comparative studies are facilitated. MTTBs containing miscellaneous neoplasms are suitable for screening of hybridoma supernatants during the early phases of monoclonal antibody production. This permits economical and rapid screening for tissue-specific monoclonal antibodies and simultaneous selection of those that perform well in tissue sections. MTTBs are also useful for the routine monitoring of sensitivity in immunohistochemistry because they can be prepared to include tissues with a broad spectrum of antigen densities. Because a large number of nearly identical sections can be obtained from a single MTTB, these sections could be useful for large scale interlaboratory quality control studies.
我们描述了一种将100个或更多不同组织样本包埋在常规大小石蜡块中的方法，即多肿瘤组织块（MTTB）。MTTB可使用一滴抗体在一张载玻片上对众多组织样本同时进行免疫组织学检测。由于所有组织样本在免疫染色过程中受到同等对待，大多数变异来源被消除，便于进行比较研究。含有多种肿瘤的MTTB适用于单克隆抗体生产早期阶段杂交瘤上清液的筛选。这允许经济、快速地筛选组织特异性单克隆抗体，并同时选择在组织切片中表现良好的抗体。MTTB对于免疫组织化学敏感性的常规监测也很有用，因为可以准备包含具有广泛抗原密度的组织的MTTB。由于从单个MTTB可以获得大量几乎相同的切片，这些切片可用于大规模的实验室间质量控制研究。
