溶剂前沿位置萃取及一些用于通过液相色谱-串联质谱法测定家禽饲料中抗球虫药的常规样品制备技术。
Solvent Front Position Extraction and some conventional sample preparation techniques for the determination of coccidiostats in poultry feed by LC-MS/MS.
作者信息
Rybicki Maciej Jan, Becue Ilse, Daeseleire Els, Klimek-Turek Anna, Dzido Tadeusz Henryk
机构信息
Department of Physical Chemistry, Medical University of Lublin, ul. Chodźki 4a, 20-093, Lublin, Poland.
Flanders Research Institute for Agriculture, Fisheries and Food, Technology and Food Science Unit (ILVO), Brusselsesteenweg 370, 9090, Melle, Belgium.
出版信息
Sci Rep. 2022 Mar 8;12(1):3786. doi: 10.1038/s41598-022-07587-5.
Solvent Front Position Extraction is a novel technique developed for effective sample preparation of biological samples containing coccidiostats prior to LC-MS/MS. In this study the technique was used for isolation and determination of seven coccidiostats, from both main groups being: ionophores and chemical coccidiostats. Its effectiveness was evaluated by comparing with other sample preparation procedures, used in European routine laboratories. Results obtained by Solvent Front Position Extraction were very satisfactory (linearity R ≥ 0.971, recovery 90.1-111.1%, RSDr: 8.7-16.6%, RSD: 9.0-17.7%) and fulfilled requirements described in Commission Regulation (EU) 2021/808 of 22 March 2021, which showed great potential of the technique in sample preparation of coccidiostats in poultry feed.
溶剂前沿位置萃取是一种新开发的技术，用于在LC-MS/MS分析之前对含有抗球虫药的生物样品进行有效的样品制备。在本研究中，该技术用于分离和测定七种抗球虫药，它们分属于两个主要类别：离子载体类和化学抗球虫药。通过与欧洲常规实验室使用的其他样品制备方法进行比较，评估了该技术的有效性。溶剂前沿位置萃取获得的结果非常令人满意（线性R≥0.971，回收率90.1-111.1%，相对标准偏差（RSDr）：8.7-16.6%，相对标准偏差（RSD）：9.0-17.7%），并符合2021年3月22日欧盟委员会法规（EU）2021/808中所述的要求，这表明该技术在禽饲料中抗球虫药的样品制备方面具有巨大潜力。
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