Jordan T W
Liver. 1986 Apr;6(2):78-84. doi: 10.1111/j.1600-0676.1986.tb00272.x.
The distribution of microfilaments in cryostat sections of liver from BALB/c and C57BL/6 mice was compared using the F-actin binding probe rhodaminyl phalloidin and indirect immunofluorescence using a human serum containing antiactin autoantibodies. The immunological reactivity of this serum was established by its capacity to immunoprecipitate purified skeletal muscle actin and by its ability to immunoprecipitate a protein which migrated electrophoretically with actin from 35S-labeled liver cells. Oral administration of the liver toxin sporidesmin did not substantially alter the binding of rhodaminyl phalloidin to microfilaments but the reactivity of the anti-actin serum with the liver cytoskeleton was diminished 3 h after, and enhanced within 24 h of toxin ingestion. Amounts of actin measured by DNAse inhibition were not altered. The results are assessed in terms of their significance for understanding the way in which sporidesmin causes liver damage.
使用F-肌动蛋白结合探针罗丹明鬼笔环肽，并通过含抗肌动蛋白自身抗体的人血清进行间接免疫荧光，比较了BALB/c和C57BL/6小鼠肝脏冰冻切片中微丝的分布。该血清的免疫反应性通过其免疫沉淀纯化骨骼肌肌动蛋白的能力以及免疫沉淀与来自35S标记肝细胞的肌动蛋白电泳迁移相同的蛋白质的能力得以确立。口服肝毒素鬼伞素对罗丹明鬼笔环肽与微丝的结合没有实质性改变，但在摄入毒素3小时后抗肌动蛋白血清与肝脏细胞骨架的反应性降低，而在摄入毒素24小时内反应性增强。通过DNA酶抑制法测定的肌动蛋白量没有改变。根据这些结果对理解鬼伞素导致肝损伤的方式的重要性进行了评估。
