Jordan T W, Pedersen J S
J Cell Sci. 1986 Sep;85:33-46. doi: 10.1242/jcs.85.1.33.
Changes in cell morphology and cell adhesion occurred when cultured cells from the rat liver cell strain C3 were exposed to the fungal toxins, sporidesmin or gliotoxin. Both toxins caused loss of attachment of the cells to the plastic of tissue culture plates and this effect was preceded by loss of actin cables. Other changes included cytoplasmic vacuolation and blocked entry into S-phase of the cell cycle. Under these conditions [3H]thymidine incorporation into the cells was also diminished but changes were not detected in the amount of cellular actin, or in the accessibility of cell surface proteins to iodination carried out by the Bolton and Hunter method. The observations suggest that disruption of microfilaments is one of the earliest effects of these toxins on eukaryotic cells.
当将大鼠肝细胞系C3的培养细胞暴露于真菌毒素——孢子丝菌素或胶霉毒素时，细胞形态和细胞黏附发生了变化。两种毒素均导致细胞与组织培养板塑料表面的附着丧失，且这种效应之前会出现肌动蛋白纤维束的丧失。其他变化包括细胞质空泡化以及细胞周期进入S期受阻。在这些条件下，细胞中[3H]胸腺嘧啶核苷的掺入也减少了，但未检测到细胞肌动蛋白量的变化，也未检测到通过博尔顿-亨特法进行碘化时细胞表面蛋白的可及性变化。这些观察结果表明，微丝的破坏是这些毒素对真核细胞最早产生的效应之一。
