Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China.
Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China.
Food Funct. 2022 Mar 21;13(6):3649-3659. doi: 10.1039/d1fo03391c.
As the demand of consumers for good meat quality is increasing, there is more interest in improving pork quality by nutritional regulation. Dihydromyricetin (DHM), a group of bioactive flavonoids, exhibits excellent biological functions and pharmacological activities. This study aimed to investigate whether dietary DHM supplementation in growing-finishing pigs could provide high-quality pork. A total of 24 healthy castrated Duroc × Landrace × Yorkshire (DLY) pigs with an average body weight of 26.95 ± 0.26 kg were randomly divided into four groups (basal diet and a basal diet supplemented with 100, 300 and 500 mg kg DHM) with 6 duplicates and one pig per replicate. The growth performance, carcass traits and pork quality of growing-finishing pigs were detected. Then, tandem mass tag (TMT) based quantitative proteomics, western blotting and real-time quantitative polymerase chain reaction (RT-qPCR) were used to explore the regulatory mechanism of DHM on pork quality. The results showed that DHM decreased the feed to gain ratio (F/G) and improved the sensory quality (shear force and meat color) and nutritional value (crude protein content and amino acid composition) of pork in growing-finishing pigs. Totally 22 differentially expressed proteins were identified, among which the down-regulated heat shock protein-β1 (HSPB1) and up-regulated Troponin C-slow (TNNC1) contributed towards explaining the positive effect of DHM on the tenderness and meat color of pork. GO enrichment analysis revealed that better meat color was also linked to higher levels of oxidative metabolism and hemoglobin complexes in pork. Western blotting and RT-qPCR analysis showed that DHM induced a muscle fiber type transformation from fast-switch to slow-switch by activating the AMP-activated protein kinase (AMPK) signal, thereby improving the pork quality. Taken together, our findings provide effective evidence for the application of DHM in high-quality pork production.
随着消费者对优质肉质的需求不断增加，通过营养调控来改善猪肉品质的兴趣日益浓厚。二氢杨梅素（DHM）是一类具有生物活性的黄酮类化合物，具有优异的生物学功能和药理活性。本研究旨在探讨在生长育肥猪日粮中添加 DHM 是否可以提供优质猪肉。将 24 头健康去势杜洛克×长白×大约克（DLY）猪，平均体重为 26.95±0.26kg，随机分为 4 组（基础日粮和基础日粮分别添加 100、300 和 500mg/kgDHM），每组 6 个重复，每个重复 1 头猪。检测生长育肥猪的生长性能、胴体性状和猪肉品质。然后，采用串联质量标签（TMT）定量蛋白质组学、Westernblotting 和实时定量聚合酶链反应（RT-qPCR）技术，探讨 DHM 对猪肉品质的调控机制。结果表明，DHM 降低了料重比（F/G），提高了生长育肥猪猪肉的感官品质（剪切力和肉色）和营养价值（粗蛋白含量和氨基酸组成）。共鉴定出 22 个差异表达蛋白，其中下调的热休克蛋白-β1（HSPB1）和上调的肌钙蛋白 C-慢（TNNC1）对 DHM 改善猪肉嫩度和肉色的积极作用有贡献。GO 富集分析表明，猪肉更好的肉色也与更高水平的氧化代谢和血红蛋白复合物有关。Western blotting 和 RT-qPCR 分析表明，DHM 通过激活 AMP 激活蛋白激酶（AMPK）信号，诱导肌纤维类型从快转换向慢转换，从而改善猪肉品质。综上所述，本研究为 DHM 在优质猪肉生产中的应用提供了有效的证据。
