Henkel AG & Co. KGaA, Düsseldorf, Germany.
Department of Pharmacy, Institute for Pharmaceutical and Medicinal Chemistry, Heinrich Heine University, Düsseldorf, Germany.
Int J Cosmet Sci. 2022 Apr;44(2):216-231. doi: 10.1111/ics.12769. Epub 2022 Mar 31.
Human eccrine sweat glands (eSG) represent vital components of the skin involved in regulating body temperature. Especially the eccrine duct, which opens directly into the skin surface and releases the aqueous sweat, constitutes the first contact point with topically applied substances. For scientific investigations and to understand the underlying sweating mechanism on a cellular level defined cellular material is beneficial. We, therefore, strived to generate a cell line derived from human eSG duct cells for identifying new mechanisms in sweating control, as such a standardized cell line is currently lacking.
Isolated primary human eSG duct cells were transduced with simian virus 40 large T antigen (SV40T) by lentiviral transduction. Successfully SV40T-transduced clones were selected by single-cell cloning with one clone, named 1D10, being particularly described in this work.
In performed cellular investigations, SV40T-transduced duct-derived cells exhibited an extended lifespan with stable population doubling times suggesting its immortality. Besides, 1D10 clonal cell culture demonstrated similarities with parental, primary duct cells regarding gene expression of selected sweat gland-related markers. When combined with primary coil cells in a hanging drop co-culture, those transduced duct-derived cells showed some duct cell-like features. Further, a certain degree of cellular communication and a specific reaction towards substance application was observed.
Generated and herein described cell line derived from isolated human eSG duct cells is, based on the presented scientific findings, considered as immortal. Besides, this cell line shows some similarity with primary duct cells, although alterations from native glands were detected, among which is loss of expression of cystic fibrosis transmembrane conductance regulator. Provided some further investigations, presented SV40T-transduced duct-cell derived cell line seems a suited surrogate of primary eccrine duct cells.
人汗腺（eSG）是皮肤中参与调节体温的重要组成部分。特别是直接开口于皮肤表面并释放水性汗液的外分泌管，构成了与局部应用物质的第一个接触点。为了进行科学研究并了解细胞水平上出汗的潜在机制，使用定义明确的细胞材料是有益的。因此，我们努力从人外分泌管细胞中生成细胞系，以鉴定出汗控制的新机制，因为目前缺乏标准化的细胞系。
通过慢病毒转导将猿猴病毒 40 大 T 抗原（SV40T）转导到分离的原代人外分泌管细胞中。通过单细胞克隆选择成功转导 SV40T 的克隆，其中一个克隆，命名为 1D10，在本工作中特别描述。
在进行的细胞研究中，SV40T 转导的导管衍生细胞表现出延长的寿命和稳定的倍增时间，表明其具有永生性。此外，1D10 克隆细胞培养在选择的汗腺相关标记物的基因表达方面与亲本原代导管细胞具有相似性。当与原代细胞在悬滴共培养中结合时，那些转导的导管衍生细胞表现出一些导管细胞样特征。此外，观察到一定程度的细胞通讯和对物质应用的特定反应。
根据目前的科学发现，从分离的人外分泌管细胞中生成和描述的细胞系被认为是永生的。此外，尽管从天然腺体中检测到一些改变，包括囊性纤维化跨膜电导调节剂表达的丧失，但该细胞系与原代导管细胞具有某些相似性。提供了一些进一步的研究，提出的 SV40T 转导的导管细胞衍生细胞系似乎是原代外分泌导管细胞的合适替代物。
