Department of Biology, Science Faculty, Selcuk University, 42130, Konya, Turkey.
Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Réduit, 80837, Mauritius.
Chem Biodivers. 2022 Apr;19(4):e202200076. doi: 10.1002/cbdv.202200076. Epub 2022 Mar 24.
In this study, phytochemical and pharmacological screening of the aerial part and roots extracts from Doronicum orientale Hoffm. (Asteraceae) was carried out. Plant extracts were obtained using solvents of different polarity (hexane, ethyl acetate, ethanol, ethanol/water, water) for selection the most optimal solvent for the extraction of active compounds. For instance, the extracts yielded total phenolic and flavonoid contents in the range of 12.13-45.67 mg GAE/g and 0.75-12.44 mg QE/g, respectively, while the total antioxidant capacity of the extracts determined by the phosphomolybdenum assay ranged from 0.88-2.53 mmol TE/g. HPLC/MS/MS analysis revealed 5-caffeoylquinic acid (2.52-337.05 μg/g) and 3,5-dicaffeoylquinic acid (3.12-299.36 μg/g) to be the major components present in the investigated extracts. Antioxidant activity in terms of radical scavenging ability of the extracts ranged from 0.82-45.56 mg TE/g in DPPH assay and from 5.07-104.58 mg TE/g in ABTS assay. The tested extracts were found to inhibit acetylcholinesterase (aerial part: 0.50-2.33 mg GALAE/g; roots: 0.40-2.43 mg GALAE/g), while with the exception of the water extracts, the other extracts showed butyrylcholinesterase inhibition (aerial part: 2.46-5.02 mg GALAE/g; root: 2.93-4.17 mg GALAE/g). Overall, this study presented an interesting scope of this species in phytomedicine with preliminary data demonstrating some of the tested extracts to possess high bioactive contents, antioxidant potential and enzyme inhibitory activity. Thus, additional investigations are necessary to confirm their safety in herbal drug applications.
本研究对东方缬草（菊科）地上部分和根提取物进行了植物化学和药理学筛选。使用不同极性的溶剂（正己烷、乙酸乙酯、乙醇、乙醇/水、水）获得植物提取物，以选择最适合提取活性化合物的溶剂。例如，提取物的总酚和类黄酮含量范围分别为 12.13-45.67mgGAE/g 和 0.75-12.44mgQE/g，而提取物的总抗氧化能力通过磷钼酸盐测定法测定范围为 0.88-2.53mmolTE/g。HPLC/MS/MS 分析表明，5-咖啡酰奎宁酸（2.52-337.05μg/g）和 3,5-二咖啡酰奎宁酸（3.12-299.36μg/g）是在所研究的提取物中存在的主要成分。提取物的抗氧化活性以清除自由基能力表示，在 DPPH 测定法中范围为 0.82-45.56mgTE/g，在 ABTS 测定法中范围为 5.07-104.58mgTE/g。测试提取物被发现抑制乙酰胆碱酯酶（地上部分：0.50-2.33mgGALAE/g；根：0.40-2.43mgGALAE/g），而除水提取物外，其他提取物显示出丁酰胆碱酯酶抑制作用（地上部分：2.46-5.02mgGALAE/g；根：2.93-4.17mgGALAE/g）。总的来说，这项研究为该物种在植物医学中的应用提供了有趣的研究前景，初步数据表明一些测试提取物具有高生物活性含量、抗氧化潜力和酶抑制活性。因此，需要进一步的研究来确认它们在草药药物应用中的安全性。
