Franzén G, Olofsson J, Risberg B, Klintenberg C, Nordenskjöld B
Pathol Res Pract. 1986 May;181(2):230-5. doi: 10.1016/S0344-0338(86)80015-2.
DNA measurements were performed on formalin-fixed, paraffin-embedded squamous cell carcinomas, using the method of Hedley et al. Ultrasonication was sometimes necessary after enzymatic disintegration to dispose of residual cytoplasm. The isolated nuclei were stained with Hoechst 33258. DNA measurements were performed using a rapid system for static fluorometry (FLUORA-programme). The method proved to be highly accurate. The mean CV of the stem cell peaks from different tumours was 5.3. There was no difference between material less or more than 5 years old (mean CVs 5.2 and 5.5 respectively). RNase treatment had no effect and was therefore not considered necessary. The proliferative activity was calculated by computer assuming a rectilinear distribution of S-phase cells. Because routinely paraffin-embedded material can be used, DNA analysis can be performed on tumour material where the clinical outcome is known, in order to evaluate certain DNA variables.
采用Hedley等人的方法，对福尔马林固定、石蜡包埋的鳞状细胞癌进行DNA测量。酶解后有时需要超声处理以去除残留的细胞质。分离出的细胞核用Hoechst 33258染色。使用快速静态荧光测定系统（FLUORA程序）进行DNA测量。该方法被证明具有高度准确性。不同肿瘤干细胞峰的平均变异系数为5.3。保存时间少于或多于5年的材料之间没有差异（平均变异系数分别为5.2和5.5）。核糖核酸酶处理没有效果，因此认为没有必要。假定S期细胞呈直线分布，通过计算机计算增殖活性。由于可以使用常规石蜡包埋材料，因此可以对已知临床结果的肿瘤材料进行DNA分析，以评估某些DNA变量。
