Weger A R, Mikuz G, Askensten U, Auer G U, Schwab G, Glaser K S
Department of Pathology, University of Innsbruck, Austria.
Pathol Res Pract. 1989 Nov;185(5):752-4. doi: 10.1016/S0344-0338(89)80232-8.
Deparaffinized and disintegrated material from conventionally formalin-fixed and paraffin-embedded surgical specimens of 100 cases of ductal adenocarcinoma of the pancreas was Feulgen-stained, and the cytochemical DNA distribution patterns of at least 100 single tumour cells and 50 "control" cells (fibrocytes) were assessed by means of image cytometry (ICM). In 77 cases a sufficient number of neoplastic cells could be obtained for these DNA assessments. The fairly high number (23) of cases that had to be excluded due to too small amounts of disintegrated cells or cell nuclei may be explained by the high content of connective tissue stroma in these pancreatic adenocarcinomas. The tumour cell nuclei in 76 of these 77 cases showed cytochemically a clear-cut "non-diploid" DNA distribution pattern. This observation reflects the well-known highly malignant growth potential of this carcinoma. Despite the fact that about 1/4 of the tumours had to be excluded, the main result of our methodological study is, after all that conventionally formalin-fixed paraffin-embedded specimens of most pancreatic adenocarcinomas can be successfully used for the deparaffinization-disintegration procedure preceding the nuclear DNA assessments by means of ICM. Additional studies are, however, required to obtain the diagnostic and prognostic impact of the results of such cytochemical analyses of the DNA distribution pattern in adenocarcinomas of the pancreas.
对100例胰腺导管腺癌的传统福尔马林固定石蜡包埋手术标本进行脱蜡和细胞解离处理，然后进行Feulgen染色，并通过图像细胞术（ICM）评估至少100个单个肿瘤细胞和50个“对照”细胞（纤维细胞）的细胞化学DNA分布模式。在77例病例中，能够获得足够数量的肿瘤细胞用于这些DNA评估。有23例病例因解离细胞或细胞核数量过少而不得不排除，这可能是由于这些胰腺腺癌中结缔组织基质含量较高所致。在这77例病例中的76例中，肿瘤细胞核在细胞化学上显示出明确的“非二倍体”DNA分布模式。这一观察结果反映了这种癌症众所周知的高度恶性生长潜能。尽管约四分之一的肿瘤不得不被排除，但我们方法学研究的主要结果是，大多数胰腺腺癌的传统福尔马林固定石蜡包埋标本毕竟可以成功用于在通过ICM进行核DNA评估之前的脱蜡 - 细胞解离程序。然而，还需要进一步的研究来确定胰腺腺癌DNA分布模式的这种细胞化学分析结果的诊断和预后意义。
