Mie Axel, Novak Vlastimil, Franko Mikael Andersson, Bügel Susanne Gjedsted, Laursen Kristian Holst
Department of Clinical Science and Education, Södersjukhuset, Karolinska Institutet, Stockholm 11883, Sweden.
Department of Environmental Science, Stockholm University, Stockholm 106 91, Sweden.
J Agric Food Chem. 2022 Mar 23;70(11):3391-3399. doi: 10.1021/acs.jafc.1c04418. Epub 2022 Mar 9.
The stable nitrogen isotope ratio δN is used as a marker of dietary protein sources in blood. Crop fertilization strategies affect δN in plant foods. In a double-blinded randomized cross-over dietary intervention trial with 33 participants, we quantified the effect of fertilizer type (conventional: synthetic fertilizer and organic: animal or green manure) on δN in blood plasma. At study baseline, plasma δN was +9.34 ± 0.29‰ (mean ± standard deviation). After 12 days intervention with a diet based on crops fertilized with animal manure, plasma δN was shifted by +0.27 ± 0.04‰ (mean ± standard error) compared to synthetic fertilization and by +0.22 ± 0.04‰ compared to fertilization with green manure (both < 0.0001). Accordingly, differences in the δN values between fertilizers are propagated to the blood plasma of human consumers. The results indicate a need to consider agricultural practices when using δN as a dietary biomarker.
稳定氮同位素比率δN被用作血液中膳食蛋白质来源的标志物。作物施肥策略会影响植物性食物中的δN。在一项有33名参与者的双盲随机交叉饮食干预试验中，我们量化了肥料类型（传统肥料：合成肥料；有机肥料：动物粪便或绿肥）对血浆中δN的影响。在研究基线时，血浆δN为+9.34±0.29‰（平均值±标准差）。在用以动物粪便施肥的作物为基础的饮食进行12天干预后，与合成肥料相比，血浆δN升高了+0.27±0.04‰（平均值±标准误），与绿肥施肥相比升高了+0.22±0.04‰（两者均P<0.0001）。因此，不同肥料之间δN值的差异会传递给人类消费者的血浆。结果表明，在将δN用作膳食生物标志物时需要考虑农业实践。
