Wang Ziquan, Wang Meng, Xu Qian, Liu Siyuan, Gao Ying, Chang Haiyan, Sui Zhiwei
Center for Advanced Measurement Science, National Institute of Metrology, Beijing, China.
College of Life Sciences, Hunan Normal University, Changsha, China.
Foodborne Pathog Dis. 2022 Apr;19(4):272-280. doi: 10.1089/fpd.2021.0088. Epub 2022 Mar 9.
, O157, and are typical foodborne pathogens in ground beef, which can cause severe infection even when present as a single cell. Flow cytometry (FCM) methods are widely applied in the rapid detection of pathogens in food products. In this study, we report an FCM-based method for detecting single cells of , O157, and . in 25 g ground beef samples. We fluorescently labeled specific antibodies that could effectively identify bacterial cells, prepared single-cell samples by serial dilution, and optimized the pre-enrichment time. The results showed that 7 h of pre-enrichment is appropriate for sensitive single-cell detection by FCM. Finally, we evaluated this method in artificially contaminated and retail beef samples. This study outlines a novel highly sensitive FCM-based method to detect , O157, and . in beef samples within 8 h that can be applied to the rapid and multiplexed detection of foodborne pathogens.
，O157和 是碎牛肉中典型的食源性病原体，即使以单个细胞形式存在也可导致严重感染。流式细胞术（FCM）方法广泛应用于食品中病原体的快速检测。在本研究中，我们报告了一种基于FCM的方法，用于检测25克碎牛肉样品中的 、O157和 的单个细胞。我们对可有效识别细菌细胞的特异性抗体进行荧光标记，通过系列稀释制备单细胞样品，并优化了预富集时间。结果表明，7小时的预富集时间适合通过FCM进行灵敏的单细胞检测。最后，我们在人工污染和零售牛肉样品中评估了该方法。本研究概述了一种基于FCM的新型高灵敏度方法，可在8小时内检测牛肉样品中的 、O157和 ，该方法可应用于食源性病原体的快速多重检测。
