Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China.
Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China; College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
Talanta. 2022 Feb 1;238(Pt 1):123005. doi: 10.1016/j.talanta.2021.123005. Epub 2021 Nov 4.
Salmonella, Escherichia coli O157:H7 (E. coli O157:H7) and Shigella flexneri (S. flexneri) might contaminate similar types of meat products and cause deadly diseases in humans. In reality, ground beef samples may carry more than one pathogen and a rapid and accurate detection method for the simultaneous identification of multiple specific pathogenic strains in ground beef is crucial. In this study, a sample pretreatment protocol and a flow cytometry method were developed for rapid and multiplexed quantification of the three pathogens without cultural enrichment in ground beef. The whole process of sample pretreatment, staining, and instrument analysis can be accomplished within 1 h. The three bacteria upon sample pretreatment were demonstrated good recoveries (93.8%-101.2%). The quantitative detection range of the mothed was 10 to 10 cells/g for all three pathogens, and the detection limit for Salmonella, E. coli O157:H7 and S. flexneri in ground beef were 3.1 × 10 cells/g, 2.1 × 10 cells/g and 2.3 × 10 cells/g, respectively. Therefore, the as-developed approach is a rapid and quantitative method for multiplexed detection of Salmonella, E. coli O157:H7, and S. flexneri in ground beef.
沙门氏菌、O157:H7 型大肠杆菌（E. coli O157:H7）和福氏志贺菌（S. flexneri）可能污染类似类型的肉类产品，并导致人类致命疾病。实际上，绞碎牛肉样本可能携带不止一种病原体，因此快速、准确地检测绞碎牛肉中多种特定病原体的方法至关重要。在本研究中，开发了一种样品预处理方案和一种流式细胞术方法，用于在不进行培养富集的情况下快速、多重定量检测绞碎牛肉中的三种病原体。整个样品预处理、染色和仪器分析过程可在 1 小时内完成。经样品预处理后，三种细菌的回收率均良好（93.8%-101.2%）。该方法的定量检测范围为所有三种病原体的 10 到 10 个细胞/g，在绞碎牛肉中，沙门氏菌、E. coli O157:H7 和 S. flexneri 的检测限分别为 3.1×10 个细胞/g、2.1×10 个细胞/g 和 2.3×10 个细胞/g。因此，所开发的方法是一种快速、定量的多重检测绞碎牛肉中沙门氏菌、E. coli O157:H7 和 S. flexneri 的方法。
