Department of Pathology, University of Medicine and Pharmacy of Craiova, Romania;
Rom J Morphol Embryol. 2021 Jul-Sep;62(3):705-712. doi: 10.47162/RJME.62.3.07.
Epithelial-mesenchymal transition (EMT) is an essential biological process involved in the initiation and progression of cancer by which epithelial tumor cells lose their differentiated characteristics, such as cell-cell adhesion and apical-basal polarity and acquire a more invasive and∕or metastatic mesenchymal phenotype. The present study investigated the expression of immunomarkers with a role in EMT of non-melanoma skin cancers (NMSCs), such as E-cadherin, fibronectin and Slug, for a number of 50 NMSCs, represented by 30 cases of basal cell carcinomas (BCCs) and 20 cases of squamous cell carcinomas (SCCs). For BCC, the statistical analysis of the investigated immunomarkers indicated significantly differences in relation to the depth of invasion, and for E-cadherin and fibronectin with the degree of risk. In the case of SCC, the statistical analysis indicated significant differences of E-cadherin and Slug with the degree of tumor differentiation, and for fibronectin and Slug with the depth of invasion. The analysis of the distribution for the percentage values of the investigated immunomarkers in the case of BCC indicated a significant negative linear relation between E-cadherin/fibronectin and E-cadherin/Slug, and in SCC a significant negative linear relation between E-cadherin∕fibronectin, E-cadherin∕Slug and a positive linear one in the case of fibronectin∕Slug. The study indicates through the statistically significant relation between E-cadherin∕fibronectin and E-cadherin∕Slug, the EMT intervention in carcinogenesis of NMSC.
上皮-间充质转化 (EMT) 是癌症发生和发展过程中的一个重要生物学过程，在此过程中，上皮肿瘤细胞失去其分化特征，如细胞-细胞黏附性和顶端-基底极性，并获得更具侵袭性和/或转移性的间质表型。本研究调查了与非黑色素瘤皮肤癌 (NMSC) 的 EMT 相关的免疫标志物的表达，如 E-钙黏蛋白、纤连蛋白和 Slug，共涉及 50 例 NMSC，其中 30 例为基底细胞癌 (BCC)，20 例为鳞状细胞癌 (SCC)。对于 BCC，对所研究的免疫标志物的统计分析表明，与浸润深度有关的差异具有统计学意义，而与风险程度有关的 E-钙黏蛋白和纤连蛋白具有统计学意义。对于 SCC，统计分析表明，E-钙黏蛋白和 Slug 与肿瘤分化程度有关，纤连蛋白和 Slug 与浸润深度有关。在 BCC 情况下，对所研究的免疫标志物的百分比值分布进行分析，结果表明 E-钙黏蛋白/纤连蛋白和 E-钙黏蛋白/Slug 之间呈显著负线性关系，在 SCC 中，E-钙黏蛋白/纤连蛋白、E-钙黏蛋白/Slug 之间呈显著负线性关系，而纤连蛋白/Slug 之间呈显著正线性关系。研究表明，通过 E-钙黏蛋白/纤连蛋白和 E-钙黏蛋白/Slug 之间具有统计学意义的关系，提示 EMT 参与了 NMSC 的癌变过程。
