Dobriţoiu Maria, Stepan Alex Emilian, Mărgăritescu Claudiu, Simionescu Cristiana Eugenia, Vere Cristin Constantin, Schenker Michael, Crişan Anda Elena
Department of Pathology, University of Medicine and Pharmacy of Craiova, Romania;
Rom J Morphol Embryol. 2019;60(2):573-579.
One of the mechanisms involved in gastric carcinomas progression is represented by epithelial-mesenchymal transition (EMT), a complex process during which tumor cells acquire an invasive and migratory mesenchymal phenotype. In this study, we analyzed the immunoexpression of E-cadherin, P-cadherin and fibronectin in 50 gastric carcinomas, in relation with the tumoral type, differentiation grade and lesions stage. The reactions presented variable patterns related to lesions stage. Membrane and cytoplasmic reactions were present in 62% of cases for E-cadherin and in 56% of cases for P-cadherin, being present only cytoplasmic in 34% of cases for fibronectin. The immunoexpression for E-cadherin and P-cadherin was superior in tubular gastric carcinomas, of low grade and early stage, while fibronectin expression was superior in discohesive or mixed gastric carcinomas, of high grade and in advanced stages. Negative E-∕P-cadherin and positive fibronectin immunophenotype may be associated with aggressive gastric carcinomas and supports the EMT involvement in gastric carcinogenesis.
上皮-间质转化（EMT）是胃癌进展过程中涉及的机制之一，这是一个复杂的过程，在此过程中肿瘤细胞获得侵袭性和迁移性的间质表型。在本研究中，我们分析了50例胃癌中E-钙黏蛋白、P-钙黏蛋白和纤连蛋白的免疫表达，及其与肿瘤类型、分化程度和病变分期的关系。这些反应呈现出与病变分期相关的不同模式。E-钙黏蛋白的膜反应和胞质反应出现在62%的病例中，P-钙黏蛋白的膜反应和胞质反应出现在56%的病例中，而纤连蛋白仅在34%的病例中出现胞质反应。E-钙黏蛋白和P-钙黏蛋白的免疫表达在低级别、早期的管状胃癌中较高，而纤连蛋白表达在高级别、晚期的分散性或混合型胃癌中较高。E-钙黏蛋白/ P-钙黏蛋白阴性和纤连蛋白阳性免疫表型可能与侵袭性胃癌相关，并支持EMT参与胃癌的发生发展。
