Owosho Adepitan A, Adesina Olufunlola M, Odujoko Oluwole, Akinyemi Hezekiah, Komolafe Akinwumi, Tadros Simon, Bauer Robert, Summersgill Kurt F
Missouri School of Dentistry and Oral Health, A.T. Still University Kirksville, Missouri, United States.
Department of Oral and Maxillofacial Surgery and Oral Pathology, Faculty of Dentistry, Obafemi Awolowo University Ile-Ife, Nigeria.
Int J Clin Exp Pathol. 2022 Feb 15;15(2):63-71. eCollection 2022.
The main oncologic events in pleomorphic adenoma (PA) are the translocations of () on chromosome 8q12 and () on chromosome 12q14.3 with various fusion partners. These translocations result in the transcriptional up-regulation of PLAG1 and HMGA2 proteins. We carried out a preliminary evaluation of translocation by fluorescence in-situ hybridization (FISH), immunohistochemistry (IHC) and HMGA2 IHC on twenty-five archived formalin-fixed paraffin-embedded tissues of PAs and its clinicopathologic features. Only eight cases were successfully hybridized and 50% of the interpretable cases were considered positive for translocation. PLAG1 IHC was only positive in 2 (8%) of the 25 cases stained, including one of the positive translocation cases. HMGA2 IHC was positive in 12 (48%) of the 25 cases stained including 2 (50%) of the 4 cases identified with translocation by FISH, 3 (75%) of the 4 cases negative for translocation by FISH and 7 (41%) of the 17 cases with failed hybridization. Overall, 15 (60%) of the 25 PA cases demonstrated and/or alterations confirmed either by FISH or IHC. In conclusion, and alterations were confirmed either by FISH or IHC in this cohort and alteration is a common event in PAs of salivary gland.
多形性腺瘤（PA）中的主要肿瘤学事件是8号染色体q12上的（）和12号染色体q14.3上的（）与各种融合伴侣发生易位。这些易位导致PLAG1和HMGA2蛋白的转录上调。我们通过荧光原位杂交（FISH）、免疫组织化学（IHC）和HMGA2免疫组织化学对25例存档的福尔马林固定石蜡包埋的PA组织及其临床病理特征进行了易位的初步评估。仅8例成功杂交，可解释病例中有50%被认为易位阳性。在25例染色病例中，PLAG1免疫组织化学仅在2例（8%）中呈阳性，其中包括1例易位阳性病例。在25例染色病例中，HMGA2免疫组织化学在12例（48%）中呈阳性，包括FISH鉴定为易位的4例中的2例（50%）、FISH易位阴性的4例中的3例（75%）以及杂交失败的17例中的7例（41%）。总体而言，25例PA病例中有15例（60%）通过FISH或免疫组织化学证实存在和/或改变。总之，该队列中通过FISH或免疫组织化学证实了和改变，且改变是涎腺PA中的常见事件。
