Malakhova I V, Khizhniakova T M, Novokhatskiĭ A S
Tsitologiia. 1986 May;28(5):537-44.
A panel of 6 hybridomas "XEJIMA" producing monoclonal antibodies specific to HeLa cells is prepared. Monoclonal antibodies do not bind to antigens of human diploid fibroblasts, human continuous B- and T-lymphocytes and animal cell lines. The specificity of monoclonal antibodies to cellular antigens of 5 HeLa-like cell lines and 6 human tumour cells lines, not contaminated with HeLa cells, is determined. Antibody containing ascitic fluid and culture media of hybridomas XEJIMA-3, -12, -13, and -22 significantly decrease the attachment of HeLa cells to the surface of culture flasks. Monoclonal antibodies XEJIMA-11, -12 and -13 block the multiplication of HeLa cells. The effect depends on serum concentration in the nutrient medium.
制备了一组6种“XEJIMA”杂交瘤，它们产生对HeLa细胞具有特异性的单克隆抗体。单克隆抗体不与人二倍体成纤维细胞、人连续B淋巴细胞和T淋巴细胞以及动物细胞系的抗原结合。测定了单克隆抗体对5种HeLa样细胞系和6种未被HeLa细胞污染的人肿瘤细胞系的细胞抗原的特异性。含有杂交瘤XEJIMA - 3、- 12、- 13和- 22腹水和培养基的抗体显著降低HeLa细胞与培养瓶表面的附着。单克隆抗体XEJIMA - 11、- 12和- 13阻断HeLa细胞的增殖。该效应取决于营养培养基中的血清浓度。
