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[在聚酰亚胺薄膜上进行细胞培养以获得用于光学显微镜和电子显微镜研究的制剂]

[Cell cultivation on polyimide film to obtain preparations for light optical and electron microscopic research].

作者信息

Buravlev V M, Pavlova E V, Vostrikov V M

出版信息

Tsitologiia. 1986 May;28(5):587-8.

PMID:3526673
Abstract

Upon cultivation of the brain tissue and skin-muscle tissues of human embryos, a polyimide film was used as a lining. The optimal conditions of the preliminary processing of the lining (sterilization, washing) are described. An easy separation of the polyimide film from the media (in the process of tissue treatment for electron microscope studies) facilitates the use of the material for light and electron microscopy without damaging the culture.

摘要

在培养人类胚胎的脑组织和皮肤肌肉组织时,使用聚酰亚胺薄膜作为衬里。描述了衬里预处理(灭菌、清洗)的最佳条件。聚酰亚胺薄膜在介质中易于分离(在用于电子显微镜研究的组织处理过程中),这便于将该材料用于光学和电子显微镜检查,而不会损害培养物。

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