Xu Yijuan, Zhang Chuangchuang, Qi Meng, Huang Wuyang, Sui Zhongquan, Corke Harold
Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Foods. 2022 Feb 25;11(5):677. doi: 10.3390/foods11050677.
Hulless barley grass may confer many health benefits attributed to its bioactive functional components, such as polysaccharides. Here, a hot water soluble polysaccharide was extracted from hulless barley grass, and its chemical characterization and in vitro anti-cancer activities were investigated. The yield of hulless barley grass polysaccharide (HBGP) was 2.3%, and the purity reached 99.1% with a polydispersity index (PDI) of 1.11 after purification by a diethylaminoethyl cellulose (DE-32) column and an S-400 high resolution (HR) column. The molecular weight and number-average molecular weight of HBGP were 3.3 × 10 and 2.9 × 10 Da, respectively. The monosaccharide composition of HBGP included 35.1% galactose, 25.6% arabinose, 5.5% glucose, and 5.3% xylose. Based on infrared spectrum analysis, HBGP possessed pyranose and galactose residues. In addition, this water-soluble polysaccharide showed significant cell proliferation inhibitory effects against cancer cell lines HT29, Caco-2, 4T1, and CT26.WT in a dose-dependent manner, especially for HT29 (the half-inhibitory concentration IC value = 2.72 mg/mL). The results provide a basis for the development and utilization of hulless barley grass in functional foods to aid in preventing cancer.
裸大麦草可能因其生物活性功能成分（如多糖）而具有许多健康益处。在此，从裸大麦草中提取了一种热水溶性多糖，并对其化学特性和体外抗癌活性进行了研究。通过二乙氨基乙基纤维素（DE-32）柱和S-400高分辨率（HR）柱纯化后，裸大麦草多糖（HBGP）的产率为2.3%，纯度达到99.1%，多分散指数（PDI）为1.11。HBGP的分子量和数均分子量分别为3.3×10和2.9×10 Da。HBGP的单糖组成包括35.1%的半乳糖、25.6%的阿拉伯糖、5.5%的葡萄糖和5.3%的木糖。基于红外光谱分析，HBGP含有吡喃糖和半乳糖残基。此外，这种水溶性多糖对癌细胞系HT29、Caco-2、4T1和CT26.WT具有显著的细胞增殖抑制作用，且呈剂量依赖性，尤其是对HT29（半抑制浓度IC值=2.72 mg/mL）。这些结果为裸大麦草在功能性食品中的开发利用提供了依据，有助于预防癌症。
