Free-Manjarrez Samantha, Mojica Luis, Espinosa-Andrews Hugo, Morales-Hernández Norma
Tecnología Alimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A. C., CIATEJ, Unidad Zapopan, Camino Arenero 1227, El Bajío, Zapopan 45019, Mexico.
Polymers (Basel). 2022 Feb 22;14(5):854. doi: 10.3390/polym14050854.
The work aimed to develop a gel as a protective barrier of common bean protein hydrolysates to be incorporated into a Greek-style yogurt and evaluate the sensory perception and biological potential. The gel was formed by complex coacervation and induced heat at a pH 3.5 and 3:1 biopolymer ratio (whey protein and gum arabic). The gel presented a 39.33% yield, low syneresis (0.37%), and a gel strength of 100 gf. The rheological properties showed an elastic behavior (G′ > G″). The gel with the most stable characteristics favored the incorporation of 2.3 g of hydrolysates to be added into the Greek-style yogurt. Nutritionally, the Greek-style yogurt with the encapsulated hydrolysates presented 9.96% protein, 2.27% fat, and 1.76% carbohydrate. Syneresis (4.64%), titratable acidity (1.39%), and viscoelastic behavior presented similar characteristics to the Greek-style control yogurt. The bitterness and astringency in yogurt with encapsulated hydrolysates decreased 44% and 52%, respectively, compared to the yogurt control with the unencapsulated hydrolysates. The Greek-style yogurt with the encapsulated hydrolysates showed the ability to inhibit enzymes related to carbohydrate metabolism (α-amylase (92.47%) and dipeptidyl peptidase-4 (75.24%) after simulated gastrointestinal digestion). The use of gels could be an alternative to transporting, delivering, and masking off-flavors of common bean protein hydrolysates in food matrices to decrease glucose absorption for type 2 diabetes patients.
这项工作旨在开发一种凝胶,作为菜豆蛋白水解物的保护屏障,将其添加到希腊式酸奶中,并评估其感官特性和生物潜力。该凝胶通过复凝聚法形成,并在pH值为3.5、生物聚合物比例为3:1(乳清蛋白和阿拉伯胶)的条件下加热诱导而成。该凝胶的产率为39.33%,脱水收缩率低(0.37%),凝胶强度为100 gf。流变学特性显示出弹性行为(G′>G″)。具有最稳定特性的凝胶有利于将2.3 g水解物添加到希腊式酸奶中。在营养方面,添加了包封水解物的希腊式酸奶含有9.96%的蛋白质、2.27%的脂肪和1.76%的碳水化合物。脱水收缩率(4.64%)、可滴定酸度(1.39%)和粘弹性行为与希腊式对照酸奶具有相似的特征。与未包封水解物的对照酸奶相比,包封水解物的酸奶中的苦味和涩味分别降低了44%和52%。模拟胃肠道消化后,添加了包封水解物的希腊式酸奶显示出抑制与碳水化合物代谢相关酶的能力(α-淀粉酶(92.47%)和二肽基肽酶-4(75.24%))。使用凝胶可能是在食品基质中运输、递送和掩盖菜豆蛋白水解物异味的一种替代方法,以减少2型糖尿病患者的葡萄糖吸收。