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中耳炎手术期间干细胞体内分化诱导一步式乳突骨重建的研究进展

Development of Intracorporeal Differentiation of Stem Cells to Induce One-Step Mastoid Bone Reconstruction during Otitis Media Surgeries.

作者信息

Park Sung-Hee, Kim Hantai, Lee Yun Yeong, Kim Yeon Ju, Jang Jeong Hun, Choo Oak-Sung, Choung Yun-Hoon

机构信息

Department of Otolaryngology, Ajou University School of Medicine, Suwon 16499, Korea.

Department of Otorhinolaryngology, Eulji University School of Medicine, Uijeongbu 11759, Korea.

出版信息

Polymers (Basel). 2022 Feb 23;14(5):877. doi: 10.3390/polym14050877.

DOI:10.3390/polym14050877
PMID:35267699
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8912861/
Abstract

Mastoidectomy is a surgical procedure for the treatment of chronic otitis media. This study investigated the ability of rat stromal vascular fraction cells (rSVF) in combination with polycaprolactone (PCL) scaffolds and osteogenic differentiation-enhancing blood products to promote the regeneration of mastoid bone defect. Twenty male Sprague Dawley rats were randomly divided according to obliteration materials: (1) control, (2) PCL scaffold only, (3) rSVFs + PCL, (4) rSVFs + PCL + platelet-rich plasma, and (5) rSVFs + PCL + whole plasma (WP). At 7 months after transplantation, the rSVFs + PCL + WP group showed remarkable new bone formation in the mastoid. These results indicate that SVFs, PCL scaffolds, and blood products accelerate bone regeneration for mastoid reconstruction. Autologous SVF cells with PCL scaffolds and autologous blood products are promising composites for mastoid reconstruction which can be easily harvested after mastoidectomy. With this approach, the reconstruction of mastoid bone defects can be performed right after mastoidectomy as a one-step procedure which can offer efficiency in the clinical field.

摘要

乳突切除术是一种治疗慢性中耳炎的外科手术。本研究调查了大鼠基质血管成分细胞（rSVF）与聚己内酯（PCL）支架及促进成骨分化的血液制品联合使用促进乳突骨缺损再生的能力。将20只雄性Sprague Dawley大鼠根据填充材料随机分组：（1）对照组，（2）仅PCL支架组，（3）rSVF + PCL组，（4）rSVF + PCL +富血小板血浆组，以及（5）rSVF + PCL +全血（WP）组。移植后7个月，rSVF + PCL + WP组在乳突处显示出显著的新骨形成。这些结果表明，SVF、PCL支架和血液制品可加速乳突重建的骨再生。自体SVF细胞与PCL支架及自体血液制品是用于乳突重建的有前景的复合材料，在乳突切除术后可轻松获取。通过这种方法，乳突骨缺损的重建可在乳突切除术后立即作为一步手术进行，这在临床领域可提高效率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/56a5d8a4f35e/polymers-14-00877-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/de09015392c8/polymers-14-00877-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/ddf0802b16c3/polymers-14-00877-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/0b64a93c6845/polymers-14-00877-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/f6de24777698/polymers-14-00877-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/eb174c855b05/polymers-14-00877-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/56a5d8a4f35e/polymers-14-00877-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/de09015392c8/polymers-14-00877-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/ddf0802b16c3/polymers-14-00877-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/0b64a93c6845/polymers-14-00877-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/f6de24777698/polymers-14-00877-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/eb174c855b05/polymers-14-00877-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0af/8912861/56a5d8a4f35e/polymers-14-00877-g006.jpg

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本文引用的文献

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