Department of Otolaryngology, Chonnam National University Medical School, Gwangju; Research Center for Resistant Cells, Chosun Medical School, Gwangju.
Macromol Biosci. 2013 May;13(5):660-8. doi: 10.1002/mabi.201200393. Epub 2013 Feb 20.
Two different composite scaffolds, solid-freeform-fabricated PCL/β-TCP supplemented with and without collagen nanofibers are fabricated. These scaffolds are evaluated whether a combination of collagen nanofibers with PCL/β-TCP can promote osteogenesis in a mastoid obliteration. To assess the effects of the cellular activities of osteoblast-like-cells (MG63), SEM images and MTT assays are conducted. Experimental mastoid obliteration is performed using guinea pigs that are divided group A (PCL/β-TCP/collagen-nanofiber scaffold) and group B (PCL/β-TCP scaffold). The results reveal that PCL/β-TCP/collagen scaffold provide much broader cell attachment sites than PCL/β-TCP scaffold. The µ-CT and fluorescent microscopy results reveal that the acceleration of early new bone formation within the pores and scaffold itself at week 4 post-operation is more effective in group A. In addition, based on the results of the histological and µ-CT at 12 weeks post-surgery, the effective regeneration of bone in the PCL/β-TCP/collagen scaffold is appeared.
制备了两种不同的复合支架，即无定形纤维成形的 PCL/β-TCP 补充有和没有胶原纳米纤维。评估了胶原纳米纤维与 PCL/β-TCP 的组合是否可以促进乳突闭塞中的成骨作用。为了评估成骨样细胞（MG63）的细胞活性的影响，进行了 SEM 图像和 MTT 分析。使用豚鼠进行实验性乳突闭塞，将其分为 A 组（PCL/β-TCP/胶原纳米纤维支架）和 B 组（PCL/β-TCP 支架）。结果表明，PCL/β-TCP/胶原支架比 PCL/β-TCP 支架提供了更广泛的细胞附着位点。µ-CT 和荧光显微镜结果表明，在术后第 4 周，A 组中孔内和支架本身内早期新骨形成的加速更为有效。此外，根据术后 12 周的组织学和 µ-CT 结果，PCL/β-TCP/胶原支架中出现了有效的骨再生。
