氧化三甲胺及肠道微生物衍生代谢物TML和γ-丁基甜菜碱与静脉血栓栓塞症患者的血栓形成风险无关。
TMAO and Gut Microbial-Derived Metabolites TML and γBB Are Not Associated with Thrombotic Risk in Patients with Venous Thromboembolism.
作者信息
Canyelles Marina, Plaza Melania, Rotllan Noemí, Llobet Dolors, Julve Josep, Mojal Sergi, Diaz-Ricart Maribel, Soria José Manuel, Escolà-Gil Joan Carles, Tondo Mireia, Blanco-Vaca Francisco, Souto Joan Carles
机构信息
Institut de Recerca de l'Hospital Santa Creu i Sant Pau, Institut d'Investigacions Biomèdiques IIB Sant Pau, 08041 Barcelona, Spain.
Department of Clinical Biochemistry, Hospital de la Santa Creu i Sant Pau, IIB Sant Pau, 08041 Barcelona, Spain.
出版信息
J Clin Med. 2022 Mar 4;11(5):1425. doi: 10.3390/jcm11051425.
BACKGROUND
The present work evaluates the association between circulating concentrations of Trimethylamine-N-oxide (TMAO), gamma butyrobetaine (γBB), and trimetyllisine (TML) in controls and patients with venous thromboembolism (VTE) with coagulation parameters.
METHODS
The study involved 54 VTE patients and 57 controls. Platelet function, platelet hyperreactivity, platelet adhesiveness, thrombosis-associated parameters, and thrombin generation parameters were studied. Plasma TMAO, γBB, and TML determination was performed using an ultra-high-performance liquid chromatography system coupled with mass spectrometry.
RESULTS
No differences were found for TMAO, γBB, or TML concentrations between controls and VTE patients. In thrombin generation tests, TMAO, γBB, and TML showed a positive correlation with lag time and time to peak. TMAO, γBB, and TML negatively correlated with peak height. No significant differences were observed regarding TMAO, γBB, and TML concentrations between the two blood withdrawals, nor when the control and VTE patients were analyzed separately. No correlation was observed between these gut metabolites and platelet function parameters.
CONCLUSIONS
No differences were found regarding TMAO, γBB, and TML concentrations between the control and VTE groups. Some correlations were found; however, they were mild or went in the opposite direction of what would be expected if TMAO and its derivatives were related to VTE risk.
背景
本研究评估了对照组以及静脉血栓栓塞症（VTE）患者中，氧化三甲胺（TMAO）、γ-丁甜菜碱（γBB）和三甲胺赖氨酸（TML）的循环浓度与凝血参数之间的关联。
方法
该研究纳入了54例VTE患者和57例对照。研究了血小板功能、血小板高反应性、血小板黏附性、血栓形成相关参数和凝血酶生成参数。使用超高效液相色谱系统结合质谱法测定血浆中的TMAO、γBB和TML。
结果
对照组和VTE患者之间的TMAO、γBB或TML浓度未发现差异。在凝血酶生成试验中，TMAO、γBB和TML与延迟时间和达到峰值的时间呈正相关。TMAO、γBB和TML与峰值高度呈负相关。两次采血之间，以及分别分析对照组和VTE患者时，TMAO、γBB和TML浓度均未观察到显著差异。这些肠道代谢产物与血小板功能参数之间未观察到相关性。
结论
对照组和VTE组之间的TMAO、γBB和TML浓度未发现差异。发现了一些相关性；然而，这些相关性较弱，或者与如果TMAO及其衍生物与VTE风险相关时预期的方向相反。
Zotero 插件