Kowalska Marta, Bąchor Remigiusz
Faculty of Chemistry, University of Wroclaw, 50-383 Wroclaw, Poland.
Molecules. 2022 Feb 28;27(5):1601. doi: 10.3390/molecules27051601.
One effective solution in the analysis of complex mixtures, including protein or cell hydrolysates, is based on chemoselective derivatization of a selected group of compounds by using selective tags to facilitate detection. Another method is based on the capture of the desired compounds by properly designed solid supports, resulting in sample enrichment. Cysteine is one of the rarest amino acids, but at least one cysteine residue is present in more than 91% of human proteins, which clearly confirms its important role in biological systems. Some cysteine-containing peptides may serve as significant molecular biomarkers, which may emerge as key indices in the management of patients with particular diseases. In the current review, we describe recent advances in the development of cysteine-containing peptide modification techniques based on solution and solid phase derivatization and enrichment strategies.
分析包括蛋白质或细胞水解产物在内的复杂混合物的一种有效方法，是通过使用选择性标签对选定的一组化合物进行化学选择性衍生化，以促进检测。另一种方法是基于通过适当设计的固相载体捕获所需化合物，从而实现样品富集。半胱氨酸是最稀有的氨基酸之一，但超过91%的人类蛋白质中至少存在一个半胱氨酸残基，这清楚地证实了其在生物系统中的重要作用。一些含半胱氨酸的肽可能作为重要的分子生物标志物，可能成为特定疾病患者管理中的关键指标。在本综述中，我们描述了基于溶液和固相衍生化及富集策略的含半胱氨酸肽修饰技术发展的最新进展。
