Berkelman T R, Lagarias J C
Anal Biochem. 1986 Jul;156(1):194-201. doi: 10.1016/0003-2697(86)90173-9.
Biliproteins and bilipeptides subjected to discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of zinc acetate form a complex which fluoresces an orange color when viewed under ultraviolet light. The complex between the bilin chromophore and the zinc ion fluoresces at wavelengths which can be selectively visualized in gels by using a red filter. For the biliproteins phytochrome and C-phycocyanin the minimum detectable quantities are 100 and 50 ng, respectively. This is comparable to the sensitivity of Coomassie blue staining. The technique has been used for selective detection of phytochrome in plant extracts and to distinguish chromophore-bearing peptides from those not containing chromophore in proteolytic digests of phytochrome.
在乙酸锌存在的情况下，对胆色素蛋白和胆色素肽进行不连续十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，会形成一种复合物，在紫外光下观察时会发出橙色荧光。胆素发色团与锌离子之间的复合物在特定波长下发出荧光，通过使用红色滤光片可在凝胶中选择性地观察到。对于胆色素蛋白光敏色素和C-藻蓝蛋白，最低可检测量分别为100纳克和50纳克。这与考马斯亮蓝染色的灵敏度相当。该技术已用于选择性检测植物提取物中的光敏色素，并区分光敏色素蛋白水解消化产物中含有发色团的肽和不含发色团的肽。
