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噻苯隆通过调控棉花中乙烯、生长素和细胞分裂素的交叉通讯复杂性促进叶片脱落。

Thidiazuron Promotes Leaf Abscission by Regulating the Crosstalk Complexities between Ethylene, Auxin, and Cytokinin in Cotton.

机构信息

Engineering Research Center of Plant Growth Regulator, Ministry of Education/College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.

Institute of Agricultural Information, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.

出版信息

Int J Mol Sci. 2022 Feb 28;23(5):2696. doi: 10.3390/ijms23052696.

DOI:10.3390/ijms23052696
PMID:35269837
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8910847/
Abstract

Thidiazuron (TDZ) is widely used as a defoliant to induce leaf abscission in cotton. However, the underlying molecular mechanism is still unclear. In this study, RNA-seq and enzyme-linked immunosorbent assays (ELISA) were performed to reveal the dynamic transcriptome profiling and the change of endogenous phytohormones upon TDZ treatment in leaf, petiole, and abscission zone (AZ). We found that TDZ induced the gene expression of ethylene biosynthesis and signal, and promoted ethylene accumulation earlier in leaf than that in AZ. While TDZ down-regulated indole-3-acetic acid (IAA) biosynthesis genes mainly in leaf and IAA signal and transport genes. Furthermore, the IAA content reduced more sharply in the leaf than that in AZ to change the auxin gradient for abscission. TDZ suppressed CTK biosynthesis genes and induced CTK metabolic genes to reduce the IPA accumulation for the reduction of ethylene sensitivity. Furthermore, TDZ regulated the gene expression of abscisic acid (ABA) biosynthesis and signal and induced ABA accumulation between 12-48 h, which could up-regulate ABA response factor genes and inhibit IAA transporter genes. Our data suggest that TDZ orchestrates metabolism and signal of ethylene, auxin, and cytokinin, and also the transport of auxin in leaf, petiole, and AZ, to control leaf abscission.

摘要

噻苯隆（TDZ）被广泛用作落叶剂，以诱导棉花叶片脱落。然而，其潜在的分子机制仍不清楚。本研究通过 RNA-seq 和酶联免疫吸附测定（ELISA），揭示了 TDZ 处理叶片、叶柄和离区（AZ）中动态转录组谱和内源性植物激素变化。我们发现，TDZ 诱导乙烯生物合成和信号的基因表达，并在叶片中比在 AZ 更早地促进乙烯积累。而 TDZ 下调吲哚-3-乙酸（IAA）生物合成基因主要在叶片中，IAA 信号和运输基因。此外，IAA 含量在叶片中比在 AZ 中急剧减少，以改变脱落的生长素梯度。TDZ 抑制细胞分裂素（CTK）生物合成基因，并诱导 CTK 代谢基因减少 IPA 积累，以降低乙烯敏感性。此外，TDZ 调节脱落酸（ABA）生物合成和信号的基因表达，并在 12-48 小时之间诱导 ABA 积累，这可能上调 ABA 反应因子基因并抑制 IAA 转运体基因。我们的数据表明，TDZ 协调乙烯、生长素和细胞分裂素的代谢和信号，以及生长素在叶片、叶柄和 AZ 中的运输，以控制叶片脱落。

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