He Wei, Chai Qiang, Zhang Dan, Li Wenli, Zhao Cai, Yin Wen, Fan Hong, Yu Aizhong, Hu Falong, Fan Zhilong
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070 People's Republic of China.
College of Agronomy, Gansu Agricultural University, Lanzhou, 730070 People's Republic of China.
Physiol Mol Biol Plants. 2023 Apr;29(4):513-523. doi: 10.1007/s12298-023-01309-5. Epub 2023 May 1.
Artificial light application is an effective method for promoting potato production in indoor facilities. In this study, we assessed the effects of different combinations of red (R) and blue (B) light application on potato leaf and tuber growth. Potato plantlets were transplanted under W (white light, control), RB (50% R + 50% B), RB (30% R + 70% B to 70% R + 30% B) and RB (10% R + 90% B to 90% R + 10% B), and ascorbic acid (AsA) metabolism in leaves and cytokinin (CTK), auxin (indole-3-acetic acid, IAA), abscisic acid (ABA), and gibberellin (GA) levels in tubers were measured. At 50 days of treatment, potato leaves had significantly higher L-galactono-1,4-lactone dehydrogenase (GalLDH) activity and utilized AsA faster under RB treatment than under RB treatment. CTK/IAA and ABA/GA ratios in large tubers under W treatment did not differ significantly from those under RB treatment, which had higher levels than those under RB and RB treatment at 50 days. However, under RB treatment, total leaf area decreased rapidly from 60 to 75 days compared with plants under RB treatment. Tuber dry weight per plant under W and RB treatment approached a plateau at 75 days. At 80 days, RB treatment significantly improved ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase activity compared with RB treatment. RB treatment with a high ratio of blue light increased CTK/IAA and ABA/GA to improve tuber bulking at 50 days, while RB treatment with a high ratio of red light stimulated AsA metabolic pathway to delay leaf oxidation and maintain tuber biomass accumulation at 80 days. For the indoor potato cultivation, RB treatment had a higher proportion of medium-sized tubers, thus being a suitable light treatment.
人工补光是促进设施内马铃薯生产的有效方法。在本研究中，我们评估了不同红（R）光和蓝（B）光组合对马铃薯叶片和块茎生长的影响。将马铃薯幼苗移栽到白光（W，对照）、RB（50%R + 50%B）、RB（30%R + 70%B至70%R + 30%B）和RB（10%R + 90%B至90%R + 10%B）条件下，并测定叶片中的抗坏血酸（AsA）代谢以及块茎中的细胞分裂素（CTK）、生长素（吲哚 - 3 - 乙酸，IAA）、脱落酸（ABA）和赤霉素（GA）水平。处理50天时，马铃薯叶片在RB处理下比在RB处理下具有显著更高的L - 半乳糖 - 1,4 - 内酯脱氢酶（GalLDH）活性，且利用AsA的速度更快。W处理下大薯块中的CTK/IAA和ABA/GA比值与RB处理下无显著差异，RB处理下这两个比值在50天时高于RB和RB处理。然而，在RB处理下，与RB处理的植株相比，60至75天时总叶面积迅速下降。W和RB处理下每株块茎干重在75天时接近稳定。在80天时，与RB处理相比，RB处理显著提高了抗坏血酸过氧化物酶、单脱氢抗坏血酸还原酶、脱氢抗坏血酸还原酶和谷胱甘肽还原酶的活性。高蓝光比例的RB处理在50天时提高CTK/IAA和ABA/GA以促进块茎膨大，而高红光比例的RB处理在80天时刺激AsA代谢途径以延缓叶片氧化并维持块茎生物量积累。对于室内马铃薯栽培，RB处理具有较高比例的中薯块，因此是一种合适的光照处理。
