Fiore Maria Carola, Marchese Annalisa, Mauceri Antonio, Digangi Ignazio, Scialabba Anna
Council for Agricultural Research and Economics-Research Centre for Plant Protection and Certification, S.S. 113 km 245,500, 90011 Bagheria, Italy.
Department of Agricultural, Food and Forest Sciences, University of Palermo, Viale delle Scienze-Ed. 4, 90128 Palermo, Italy.
Plants (Basel). 2022 Feb 25;11(5):631. doi: 10.3390/plants11050631.
The characterization of plant genetic resources is a precondition for genetic improvement and germplasm management. The increasing use of molecular markers for DNA-based genotype signature is crucial for variety identification and traceability in the food supply chain. We collected 75 Sicilian hazelnut accessions from private and public field collections, including widely grown varieties from the Nebrodi Mountains in north east Sicily (Italy). The germplasm was fingerprinted through nine standardized microsatellites (SSR) for hazelnut identification to evaluate the genetic diversity of the collected accessions, validating SSR discrimination power. We identified cases of homonymy and synonymy among acquisitions and the unique profiles. The genetic relationships illustrated by hierarchical clustering, structure, and discriminant analyses revealed a clear distinction between local and commercial varieties. The comparative genetic analysis also showed that the Nebrodi genotypes are significantly different from the Northern Italian, Iberian, and Turkish genotypes. These results highlight the need and urgency to preserve Nebrodi germplasm as a useful and valuable source for traits of interest employable for breeding. Our study demonstrates the usefulness of molecular marker analysis to select a reference germplasm collection of Sicilian hazelnut varieties and to implement certified plants' production in the supply chain.
植物遗传资源的特征描述是遗传改良和种质管理的前提条件。越来越多地使用基于DNA的分子标记进行基因型鉴定，对于食品供应链中的品种识别和可追溯性至关重要。我们从私人和公共田间收集了75份西西里榛子种质，包括来自意大利西西里岛东北部内布罗迪山脉广泛种植的品种。通过9个标准化微卫星（SSR）对种质进行指纹识别，以鉴定榛子，评估所收集种质的遗传多样性，验证SSR的鉴别能力。我们在采集物和独特图谱中识别出同名和同义的情况。层次聚类、结构分析和判别分析所显示的遗传关系表明，地方品种和商业品种之间存在明显差异。比较遗传分析还表明，内布罗迪基因型与意大利北部、伊比利亚和土耳其基因型有显著差异。这些结果凸显了保护内布罗迪种质的必要性和紧迫性，因为它是可用于育种的有价值的感兴趣性状来源。我们的研究证明了分子标记分析在选择西西里榛子品种参考种质库以及在供应链中实施认证植物生产方面的有用性。
