[Role of levorin and lytic enzymes in the interrelations of yeasts and actinomycete in a mixed culture].

Interrelation of yeasts and the actinomycete producing levorin, Streptomyces levoris in mixed cultures was studied. It was found that death of the accompanying microorganisms during fermentation with the two cultures was mainly due to the effect of the actinomycete lytic enzymes. The level of the enzymatic lysis depended on the genus and species characteristics of the organisms used in mixed cultures. By the end of the fermentation process the culture fluid contained no organisms with respect to which the lysoenzymes of S. levoris were highly active. In this case mixed cultivation of the actinomycete and yeasts resulted in increasing antibiotic synthesis by 45-48 per cent as compared to the control. The yeasts hydrolyzed by the S. levoris yeastlytic enzymes with difficulty or slightly remained viable up to the end of mixed cultivation and lowered the level of the antibiotic activity.