Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, 8010 Graz, Austria.
Int J Environ Res Public Health. 2022 Mar 4;19(5):2996. doi: 10.3390/ijerph19052996.
The transfer of microorganisms on packaging materials to a contact surface has only been investigated in the context of laboratory-produced spiked packaging products and agar surfaces in small quantities (0.03-0.10%) so far. Correspondingly, this study focused on the localization of microorganisms on/in industrially produced packaging materials and on the establishment of an experimental laboratory set-up to determine and quantify the parameters influencing the microbial transport from surfaces and different layers of packaging materials to contact agar media. We established a simple model to determine the transfer of microorganisms from packaging materials to microbiological agar plates. In order to clarify the transfer of microorganisms within the material, the samples were split horizontally in their z-dimension, and so produced layers (inner layers) were investigated for their microbial transfer. The parameters incubation time, applied weight and bacterial load for the samples were investigated in more detail in the outer layers (front/back) and the inner layers. No significant difference in the microbial transfer was observed between the outer and inner layers of all samples. We indicate a time-dependent transfer to the media and an independence of the transfer from the applied weight. Moreover, the number of transferred microorganisms is not dependent on the bacterial load of the samples.
到目前为止，关于包装材料上的微生物转移到接触表面的情况，仅在实验室生产的加标包装产品和少量琼脂表面（0.03-0.10%）的情况下进行了研究。因此，本研究侧重于工业生产的包装材料上微生物的定位，以及建立一个实验性实验室设置，以确定和量化影响微生物从表面和包装材料不同层转移到接触琼脂培养基的参数。我们建立了一个简单的模型来确定微生物从包装材料转移到微生物琼脂平板上的情况。为了阐明微生物在材料内部的转移，将样品在 z 方向上水平分割，并对产生的层（内层）进行微生物转移研究。在外层（正面/背面）和内层中更详细地研究了样品的孵育时间、施加的重量和细菌负荷等参数。所有样品的外层和内层之间的微生物转移没有明显差异。我们表明，微生物会随着时间的推移而转移到培养基中，并且转移与施加的重量无关。此外，转移的微生物数量与样品的细菌负荷无关。
