Scientific Operations, Waters Corporation, Milford, MA, 01757, USA.
Division of Systems Medicine, Dept Metabolism, Digestion and Reproduction, Imperial College, South Kensington, London, SW7 2AZ, UK.
J Chromatogr A. 2022 Apr 26;1669:462921. doi: 10.1016/j.chroma.2022.462921. Epub 2022 Feb 25.
The use of hybrid surface technology (HST), applied to the metal surfaces of an ACQUITY™ UPLC™ system and column, designed to mitigate the chelation, poor peak shape and analyte loss seen with acidic phospholipids was investigated. Compared to a conventional system significant improvements in both sensitivity, recovery and peak shape were obtained following UPLC on a CSH C18 column when the HST was used for the analysis of lysophosphatidic acid (LPA), phosphatidic acid (PA), lysophosphatidylserine (LPS), phosphatidylserine (PS), phosphatidylinositol-monophosphates (PIP), ceramide phosphate (CerP) and sphingoid base phosphate (SPBP). The benefits in chromatographic performance provided by the HST were seen particularly at low concentrations of these analytes. The HST system and column reduced peak tailing by 65-80% and peak width by 70-86% for LPA and PA. Moreover, increased signal intensities of up to 12.7 times were observed for LPA with the HST approach compared to the equivalent untreated LC system and column. The application of this methodology to the analysis of chicken egg PA and brain porcine PS extracts were accompanied by similar improvements in data quality.
研究了混合表面技术 (HST) 在 ACQUITY™ UPLC™ 系统和色谱柱的金属表面上的应用，旨在减轻与酸性磷脂相关的螯合作用、峰形不佳和分析物损失。与传统系统相比，在使用 HST 分析溶血磷脂酸 (LPA)、磷脂酸 (PA)、溶血磷脂丝氨酸 (LPS)、磷脂丝氨酸 (PS)、磷脂酰肌醇单磷酸 (PIP)、神经酰胺磷酸 (CerP) 和鞘氨醇碱基磷酸 (SPBP) 时，在 CSH C18 柱上进行 UPLC 后，无论是灵敏度、回收率还是峰形均得到了显著改善。在这些分析物的低浓度下，HST 提供的色谱性能优势尤为明显。HST 系统和色谱柱将 LPA 和 PA 的峰拖尾减少了 65-80%，峰宽减少了 70-86%。此外，与等效的未处理 LC 系统和色谱柱相比，HST 方法观察到 LPA 的信号强度增加了高达 12.7 倍。该方法应用于鸡卵 PA 和猪脑 PS 提取物的分析，数据质量也得到了类似的提高。
