College of Chemistry & Chemical Engineering, Central South University, Changsha 410083, PR China.
College of Chemistry & Chemical Engineering, Central South University, Changsha 410083, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jun 5;274:121096. doi: 10.1016/j.saa.2022.121096. Epub 2022 Mar 3.
As a highly toxic reagent, hydrazine (NH) is notorious to human beings and the environment. To simply and conveniently detect NH in environmental and biological systems, a ratiometric fluorescent probe MA-NH was developed with excellent sensitivity and selectivity. Probe MA-NH was readily prepared based on a naphthalene as the fluorescent scaffold and an indanedione group as the responsive moiety for NH. This probe displayed a red-emitting fluorescence at 670 nm with a large Stokes shift (200 nm). After treatment with NH, a significant blue-shifted emission at 440 nm could be observerd, which led to an extremely large emission wavelength shift (230 nm). The fluorescent intensity ratio (I/I) of probe MA-NH was rapidly and significantly increased (273-fold) within 18 min. The detection limit for NH was 0.5 µM. In addition, the probe was successfully employed for tracking NH in living cells and zebrafish through a ratiometric manner.
作为一种高毒性试剂，肼（NH）对人类和环境都有严重的危害。为了简便、快速地检测环境和生物体系中的 NH，我们开发了一种比率型荧光探针 MA-NH，它具有出色的灵敏度和选择性。探针 MA-NH 基于萘作为荧光骨架和茚满二酮基团作为 NH 的响应部分，很容易制备得到。该探针在 670nm 处发射红光，具有较大的斯托克斯位移（200nm）。与 NH 反应后，可以观察到 440nm 处明显的蓝移发射，导致发射波长发生巨大的蓝移（230nm）。探针 MA-NH 的荧光强度比（I/I）在 18 分钟内迅速且显著增加（273 倍）。NH 的检测限为 0.5µM。此外，该探针还成功地通过比率荧光法用于跟踪活细胞和斑马鱼中的 NH。
