State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment & Technology, Jiangnan University, Wuxi, Jiangsu, China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
J Hazard Mater. 2021 Mar 15;406:124589. doi: 10.1016/j.jhazmat.2020.124589. Epub 2020 Nov 28.
Isoniazid and its major metabolite, hydrazine (NH), may interfere with mitochondrial function and have negative effects on cells. Consequently, an understanding of the role of NH in mitochondria is highly desirable for protecting human health. Herein, we report a novel mitochondria-targeted ratiometric fluorescent probe (Mitro-NH) for NH detection. Mitro-NH exhibited an attenuation of green emission at 521 nm and an enhancement of yellow emission at 590 nm in the presence of NH because of hydrazinolysis, indicating that it can be used as a ratiometric chemosensor for NH with high selectivity and sensitivity. Such on-site monitoring of NH vapour using test strips and NH-moistened soil analysis demonstrated its advantages in potential application for the convenient sensing of NH. Moreover, the rationally designed probe has many potential applications for imaging NH produced in situ during the metabolism of isoniazid in living cells based on the ratio of the fluorescent signal.
异烟肼及其主要代谢物肼（NH）可能会干扰线粒体功能并对细胞产生负面影响。因此，了解 NH 在线粒体中的作用对于保护人类健康是非常可取的。在此，我们报告了一种用于 NH 检测的新型线粒体靶向比率荧光探针（Mitro-NH）。Mitro-NH 在存在 NH 的情况下，由于肼解反应，在 521nm 处的绿色发射衰减，并在 590nm 处的黄色发射增强，表明它可以用作 NH 的比率化学传感器，具有高选择性和灵敏度。使用试条和 NH 加湿土壤分析对 NH 蒸气的现场监测显示了其在方便地感应 NH 方面的潜在应用优势。此外，基于荧光信号的比值，该合理设计的探针具有许多潜在的应用，可用于在活细胞中异烟肼代谢过程中原位成像产生的 NH。
