Abbasi A, Voelter W, Zaidi Z H
Biol Chem Hoppe Seyler. 1986 May;367(5):441-5. doi: 10.1515/bchm3.1986.367.1.441.
A highly specific proteolytic enzyme cleaving at the carboxyl group of valine has been isolated from Candida tropicalis. Its specificity has been determined by digesting beta-lactoglobulin and a number of synthetic peptides. The enzyme a glycoprotein, has a molecular mass of 40 +/- 7 kDa on the basis of sodium dodecyl sulphate polyacrylamide gel electrophoresis. Its optimum activity occurs at 37 degrees C at a pH between 8-9. It has been named "Valyl-proteinase" because of its selective cleavage.
一种能在缬氨酸羧基处切割的高度特异性蛋白水解酶已从热带假丝酵母中分离出来。通过消化β-乳球蛋白和一些合成肽来确定其特异性。该酶是一种糖蛋白，根据十二烷基硫酸钠聚丙烯酰胺凝胶电泳，其分子量为40±7 kDa。其最佳活性在37℃、pH值为8至9时出现。由于其选择性切割作用，它被命名为“缬氨酰蛋白酶”。
