Purification of human plasma inactive renin by immunoaffinity chromatography on profragment-specific IgG.
作者信息
Kim S J, Hirose S, Murakami K
出版信息
Biochim Biophys Acta. 1986 Sep 5;873(1):27-30. doi: 10.1016/0167-4838(86)90185-8.
PMID:3527274
Abstract
Inactive renin was purified to apparent homogeneity from human plasma by ion exchange, gel filtration, Affi-Gel blue, immunoaffinity chromatography on profragment-specific IgG coupled to Sepharose, and preparative HPLC. By this method, a 460000-fold purification was obtained. The purified renin was totally inactive and was activated by trypsin.
摘要
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