Ikemoto F, Dzau V J, Haber E, Takaori K, Yamamoto K
Clin Sci (Lond). 1983 Aug;65(2):117-20. doi: 10.1042/cs0650117.
Canine high-molecular-weight renin (mol. wt. 60 000) is believed to be a complex of renin (low-molecular-weight form, mol. wt. 40 000) and renin-binding substance. The immunocross-reactivity of high-molecular-weight renin and low-molecular-weight renin was demonstrated by using antibodies specific to low-molecular-weight renin. Immunoaffinity chromatography with renin-specific antibodies coupled to Sepharose provided a simple and specific method for isolation of high-molecular-weight renin. High-molecular-weight renin with a specific activity of 137 600 ng of ANG I h-1 mg-1 of protein (19.6 Goldblatt units/mg of protein) was obtained. This high-molecular-weight renin was stable in dithiothreitol (25 mmol/l), suggesting that disulphide bonds may not be involved in the binding mechanism between low-molecular-weight renin and renin-binding substance. However, exposure to low pH (3.0) resulted in conversion of high-molecular-weight renin into the low-molecular-weight form.
犬高分子量肾素（分子量60000）被认为是肾素（低分子量形式，分子量40000）与肾素结合物质的复合物。使用低分子量肾素特异性抗体证明了高分子量肾素和低分子量肾素的免疫交叉反应性。用偶联到琼脂糖凝胶上的肾素特异性抗体进行免疫亲和层析提供了一种简单且特异的分离高分子量肾素的方法。获得了比活性为137600 ng ANG I h-1 mg-1蛋白质（19.6 Goldblatt单位/mg蛋白质）的高分子量肾素。这种高分子量肾素在二硫苏糖醇（25 mmol/l）中稳定，表明二硫键可能不参与低分子量肾素与肾素结合物质之间的结合机制。然而，暴露于低pH（3.0）会导致高分子量肾素转化为低分子量形式。
