Honkonen E, Näntö V, Hyörä H, Vuorinen K, Erkkola R
Biol Neonate. 1986;50(1):21-6. doi: 10.1159/000242557.
Antibacterial activity and trace element concentrations in amniotic fluid (AF) were determined in a population of 39 pregnant women in the second half of gestation. Antibacterial activity in each AF was measured by a spectrophotometric micromethod after 18 h incubation at 37 degrees C using Escherichia coli K 12 as a reference bacterium. Concentrations of zinc, iron, copper, calcium, potassium and bromine were measured by particle-induced X-ray emission method and the zinc concentration was also measured by atomic absorption spectrophotometry. Phosphate concentration was determined by direct albumin adding method. In AFs with good antibacterial activity significantly lower concentrations of potassium and bromine were found when compared to AFs with lower antibacterial activity. Concentrations of zinc, iron, copper, calcium or phosphate did not correlate with antibacterial activity in AF.
对39名处于妊娠后半期的孕妇群体的羊水（AF）中的抗菌活性和微量元素浓度进行了测定。每种羊水的抗菌活性通过分光光度微量法在37℃下使用大肠杆菌K12作为参考细菌培养18小时后进行测量。锌、铁、铜、钙、钾和溴的浓度通过粒子诱导X射线发射法测量，锌浓度也通过原子吸收分光光度法测量。磷酸盐浓度通过直接加白蛋白法测定。与抗菌活性较低的羊水相比，抗菌活性良好的羊水中钾和溴的浓度显著较低。羊水（AF）中锌、铁、铜、钙或磷酸盐的浓度与抗菌活性无关。
