Shi Wen, Zemel Dana, Sridhar Sudiksha, Mount Rebecca A, Richardson R Mark, Eden Uri T, Han Xue, Kramer Mark A, Chu Catherine J
Department of Neurology, Massachusetts General Hospital, Boston, MA, United States.
Harvard Medical School, Boston, MA, United States.
Epilepsy Behav Rep. 2022 Feb 8;18:100529. doi: 10.1016/j.ebr.2022.100529. eCollection 2022.
Epilepsy biomarkers from electroencephalogram recordings are routinely used to assess seizure risk and localization. Two widely adopted biomarkers include: (i) interictal spikes, and (ii) high frequency ripple oscillations. The combination of these two biomarkers, ripples co-occurring with spikes (spike ripples), has been proposed as an improved biomarker for the epileptogenic zone and epileptogenicity in humans and rodent models. Whether spike ripples translate to predict seizure risk in rodent seizure models is unknown. Further, recent evidence suggests ictal networks can include deep gray nuclei in humans. Whether pathologic spike ripples and seizures are also observed in the basal ganglia in rodent models has not been explored. We addressed these questions using local field potential recordings from mice with and without striatal seizures after carbachol or 6-hydroxydopamine infusions into the striatum. We found increased spike ripples in the interictal and ictal periods in mice with seizures compared to pre-infusion and post-infusion seizure-free recordings. These data provide evidence of electrographic seizures involving the striatum in mice and support the candidacy of spike ripples as a translational biomarker for seizure risk in mouse models.
脑电图记录中的癫痫生物标志物通常用于评估癫痫发作风险和定位。两种广泛采用的生物标志物包括：（i）发作间期棘波，以及（ii）高频涟漪振荡。这两种生物标志物的组合，即与棘波同时出现的涟漪（棘波涟漪），已被提议作为人类和啮齿动物模型中癫痫病灶区和癫痫源性的一种改进生物标志物。棘波涟漪是否能转化为预测啮齿动物癫痫模型中的癫痫发作风险尚不清楚。此外，最近的证据表明，发作期网络在人类中可包括深部灰质核团。在啮齿动物模型的基底神经节中是否也观察到病理性棘波涟漪和癫痫发作尚未得到探索。我们通过对向纹状体注射卡巴胆碱或6-羟基多巴胺后有或无纹状体癫痫发作的小鼠进行局部场电位记录来解决这些问题。我们发现，与注射前和注射后无癫痫发作的记录相比，有癫痫发作的小鼠在发作间期和发作期的棘波涟漪增加。这些数据提供了小鼠纹状体出现脑电图癫痫发作的证据，并支持棘波涟漪作为小鼠模型中癫痫发作风险的转化生物标志物的候选资格。
