Matsuura T, Sano Y
Cell Tissue Res. 1986;245(2):453-6. doi: 10.1007/BF00213954.
Monoaminergic nerve fibers were studied in the pineal organ of the monkey. Macaca fuscata, by use of fluorescence and immunohistochemical procedures. Abundant formations of noradrenergic nerve fibers were observed in the pineal organ. They entered the parenchyma in the form of several coarse bundles via the capsule in the distal portion of the organ and spread throughout the organ after branching into smaller units. The density of the autonomic innervation decreased gradually toward the proximal portion of the organ. In the distal portion, numerous nerve fibers formed perivascular plexuses around the blood vessels and some fibers ran as bundles unrelated to the blood vessels in the stroma. Fine varicose fibers and bundles derived from these plexuses penetrated among the pinealocytes. However, only a few intraparenchymal fluorescent fibers were detected in the proximal third of the gland. With the use of serotonin antiserum serotonin-immunoreactive nerve fibers were clearly restricted to the ventroproximal part of the pineal organ. Although the somata of the pinealocytes showed intense immunoreactivity, their processes were not stained. In one exceptional case, clusters of pinealocytes displaying very intense immunoreactivity were found in an area extending from the distal margin of the ventral portion of the pineal stalk to the proximal portion of the pineal organ proper: these cells were bipolar or multipolar and endowed with well-stained processes.
运用荧光和免疫组织化学方法，对猕猴（日本猕猴）松果体器官中的单胺能神经纤维进行了研究。在松果体器官中观察到大量去甲肾上腺素能神经纤维的形成。它们以几条粗大束状的形式通过器官远端部分的被膜进入实质，并在分支成较小单位后遍布整个器官。自主神经支配的密度朝着器官近端逐渐降低。在远端部分，大量神经纤维在血管周围形成血管周围丛，一些纤维以束状形式在基质中与血管无关地走行。源自这些丛的细的曲张纤维和束状纤维穿透松果体细胞之间。然而，在腺体近端三分之一处仅检测到少数实质内荧光纤维。使用血清素抗血清时，血清素免疫反应性神经纤维明显局限于松果体器官的腹侧近端部分。尽管松果体细胞的胞体显示出强烈的免疫反应性，但其突起未被染色。在一个特殊情况下，在从松果体柄腹侧部分的远端边缘延伸至松果体本体近端部分的区域中发现了显示非常强烈免疫反应性的松果体细胞簇：这些细胞是双极或多极的，并具有染色良好的突起。
