Clayton F, Wu J
Clin Chem. 1986 Sep;32(9):1774-7.
We compared the Abbott enzyme immunoassay method for estrogen receptor with a standard dextran-coated charcoal method, and studied the effects of treatments causing denaturation. Estrogen receptor values were slightly higher by the Abbott method, but the methods agreed with regard to receptors being present or absent in 94-98% of 50 cases. Heat lability of immunoreactivity by the Abbott enzyme immunoassay is comparable to the lability of estrogen binding by the estrogen receptor. Estrogen and molybdate substantially stabilized estrogen receptor during the assay, but improper handling of tissue before the assay may cause similar, substantial decreases in estrogen receptor by either method. The Abbott method is easier to use than the dextrancoated charcoal method, requires less tissue, and measures receptor with or without endogenously bound estrogen, but reagent cost is high.
我们将雅培酶免疫分析法用于雌激素受体检测，并与标准葡聚糖包被活性炭法进行了比较，同时研究了导致变性的处理的影响。雅培法测得的雌激素受体值略高，但在50例病例中，94% - 98%的病例中两种方法对于受体的有无判断一致。雅培酶免疫分析法中免疫反应性的热不稳定性与雌激素受体的雌激素结合不稳定性相当。在检测过程中，雌激素和钼酸盐可使雌激素受体基本稳定，但检测前对组织处理不当可能会导致两种方法测得的雌激素受体均大幅下降。雅培法比葡聚糖包被活性炭法更易于使用，所需组织更少，且可检测有无内源性结合雌激素的受体，但试剂成本较高。
