Pousette A, Gustafsson S A, Thörnblad A M, Nordgren A, Sällström J, Lindgren A, Sundelin P, Gustafsson J A
Cancer Res. 1986 Aug;46(8 Suppl):4308s-4309s.
Quantitation of estrogen receptor has been performed in cytosol prepared from 75 specimens of breast cancer tissue from patients who had not received hormonal therapy. The study was performed in order to compare an immunoassay (Abbott Laboratories, North Chicago, IL) with our currently used method for estrogen receptor analysis based on isoelectric focusing of [3H]estradiol-receptor complex in polyacrylamide gels. Using linear regression analysis, a regression coefficient (slope) of 1.30 and a correlation coefficient of 0.75 were calculated. The differences in results between the two methods are probably partly explained by the fact that the ligand-based method only measures unoccupied receptor, whereas the immunoassay detects the total amount of receptor, resulting in generally slightly higher concentrations with the latter method. However, in five of 75 specimens the ligand-based method gave a considerably higher concentration of estrogen receptor. This was most probably explained by partial proteolysis resulting in the formation of receptor fragment(s), which was undetectable with the immunoassay but detectable with the ligand-based method. These observations underline the importance of careful handling of specimens during the whole immunoassay procedure.
对75例未接受过激素治疗的乳腺癌患者的组织标本制备的胞液进行了雌激素受体定量分析。开展这项研究是为了将一种免疫测定法（美国伊利诺伊州北芝加哥雅培实验室）与我们目前使用的基于聚丙烯酰胺凝胶中[3H]雌二醇 - 受体复合物等电聚焦的雌激素受体分析方法进行比较。通过线性回归分析，计算出回归系数（斜率）为1.30，相关系数为0.75。两种方法结果的差异可能部分是由于基于配体的方法仅测量未占据的受体，而免疫测定法检测的是受体的总量，导致后一种方法测得的浓度通常略高。然而，在75个标本中有5个标本，基于配体的方法测得的雌激素受体浓度相当高。这很可能是由于部分蛋白水解导致受体片段形成，免疫测定法无法检测到，但基于配体的方法可以检测到。这些观察结果强调了在整个免疫测定过程中小心处理标本的重要性。
