• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

葡萄糖和果糖吸收的洞察及在肠上皮细胞环境中的相关性。

Insight on Glucose and Fructose Absorption and Relevance in the Enterocyte Milieu.

机构信息

Department of Agri-Food Sciences and Technologies (DISTAL), University of Bologna, Piazza Goidanich 60, 47521 Cesena, Italy.

Department of Food, Environmental and Nutritional Sciences (Defens), University of Milan, via Celoria 2, 20133 Milan, Italy.

出版信息

Nutrients. 2022 Jan 25;14(3):517. doi: 10.3390/nu14030517.

DOI:10.3390/nu14030517
PMID:35276876
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8839622/
Abstract

Although epidemiological studies indicate a strong correlation between high sugar intake and metabolic diseases, the biological mechanisms underlying this link are still controversial. To further examine the modification and crosstalk occurring in enterocyte metabolism during sugar absorption, in this study we evaluate the diffusion and intestinal metabolism of glucose, fructose and sucrose, which were supplemented in equimolar concentration to Caco-2 cells grown on polyester membrane inserts. At different time points after supplementation, changes in metabolite concentration were evaluated in the apical and basolateral chambers by nuclear magnetic resonance (NMR) and gas-chromatography (GC). Sucrose was only minimally hydrolyzed by Caco-2 cells. Upon supplementation, we observed a faster uptake of fructose than glucose, the pentose sugar being also faster catabolized. Monosaccharide absorption was concomitant to the synthesis/transport of other metabolites, which occurred differently in glucose and fructose supplemented cells. Our results confirm the prominent role of intestinal cells in fructose metabolism and clearance after absorption, representing a further step forward in the understanding of the role of dietary sugars. Future research, including targeted analysis on specific transporters/enzymes and the use of labeled substrates, will be helpful to confirm the present results and their interpretation.

摘要

尽管流行病学研究表明高糖摄入与代谢疾病之间存在很强的相关性,但这一关联的生物学机制仍存在争议。为了进一步研究在糖吸收过程中肠细胞代谢中发生的修饰和串扰,本研究评估了以等摩尔浓度补充到聚酯膜插入物上生长的 Caco-2 细胞中的葡萄糖、果糖和蔗糖的扩散和肠道代谢。在补充后的不同时间点,通过核磁共振(NMR)和气相色谱(GC)评估顶室和底室中代谢物浓度的变化。蔗糖仅被 Caco-2 细胞轻微水解。补充后,我们观察到果糖的摄取速度快于葡萄糖,戊糖的代谢速度也更快。单糖的吸收伴随着其他代谢物的合成/转运,这在葡萄糖和果糖补充的细胞中发生方式不同。我们的结果证实了肠道细胞在吸收后果糖代谢和清除中的突出作用,这是对饮食中糖的作用的进一步理解。未来的研究,包括对特定转运体/酶的靶向分析和使用标记底物,将有助于证实目前的结果及其解释。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44af/8839622/657cec6a5f1c/nutrients-14-00517-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44af/8839622/be7fa70058bc/nutrients-14-00517-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44af/8839622/d5e7a1c258d1/nutrients-14-00517-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44af/8839622/657cec6a5f1c/nutrients-14-00517-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44af/8839622/be7fa70058bc/nutrients-14-00517-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44af/8839622/d5e7a1c258d1/nutrients-14-00517-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44af/8839622/657cec6a5f1c/nutrients-14-00517-g003.jpg

相似文献

1
Insight on Glucose and Fructose Absorption and Relevance in the Enterocyte Milieu.葡萄糖和果糖吸收的洞察及在肠上皮细胞环境中的相关性。
Nutrients. 2022 Jan 25;14(3):517. doi: 10.3390/nu14030517.
2
Acute Effects of Sugars and Artificial Sweeteners on Small Intestinal Sugar Transport: A Study Using CaCo-2 Cells As an In Vitro Model of the Human Enterocyte.糖类和人工甜味剂对小肠糖转运的急性影响:一项以CaCo-2细胞作为人肠上皮细胞体外模型的研究
PLoS One. 2016 Dec 16;11(12):e0167785. doi: 10.1371/journal.pone.0167785. eCollection 2016.
3
Sugar sensing by enterocytes combines polarity, membrane bound detectors and sugar metabolism.肠上皮细胞对糖的感知结合了极性、膜结合探测器和糖代谢。
J Cell Physiol. 2007 Dec;213(3):834-43. doi: 10.1002/jcp.21245.
4
Simple-sugar meals target GLUT2 at enterocyte apical membranes to improve sugar absorption: a study in GLUT2-null mice.单糖膳食作用于肠上皮细胞顶端膜上的葡萄糖转运蛋白2(GLUT2)以改善糖吸收:一项在GLUT2基因敲除小鼠中的研究
J Physiol. 2003 Nov 1;552(Pt 3):823-32. doi: 10.1113/jphysiol.2003.049247. Epub 2003 Aug 22.
5
TNFα regulates sugar transporters in the human intestinal epithelial cell line Caco-2.TNFα 调节人肠道上皮细胞系 Caco-2 中的糖转运体。
Cytokine. 2013 Oct;64(1):181-7. doi: 10.1016/j.cyto.2013.07.004. Epub 2013 Jul 31.
6
Radiation-induced reductions in transporter mRNA levels parallel reductions in intestinal sugar transport.辐射诱导的转运体 mRNA 水平降低与肠道糖转运降低平行。
Am J Physiol Regul Integr Comp Physiol. 2010 Jan;298(1):R173-82. doi: 10.1152/ajpregu.00612.2009. Epub 2009 Nov 11.
7
Acute interactions between intestinal sugar and calcium transport in vitro.肠内糖和钙在体外的急性相互作用。
Am J Physiol Gastrointest Liver Physiol. 2014 Jan 1;306(1):G1-12. doi: 10.1152/ajpgi.00263.2013. Epub 2013 Oct 31.
8
Transport, metabolism, and endosomal trafficking-dependent regulation of intestinal fructose absorption.肠道果糖吸收的转运、代谢及内体运输依赖性调节
FASEB J. 2015 Sep;29(9):4046-58. doi: 10.1096/fj.15-272195. Epub 2015 Jun 12.
9
Insulin internalizes GLUT2 in the enterocytes of healthy but not insulin-resistant mice.胰岛素可使健康小鼠而非胰岛素抵抗小鼠的肠上皮细胞中的葡萄糖转运蛋白2(GLUT2)内化。
Diabetes. 2008 Mar;57(3):555-62. doi: 10.2337/db07-0928. Epub 2007 Dec 5.
10
Differential patterns of inhibition of the sugar transporters GLUT2, GLUT5 and GLUT7 by flavonoids.黄酮类化合物对糖转运体 GLUT2、GLUT5 和 GLUT7 的抑制作用的差异模式。
Biochem Pharmacol. 2018 Jun;152:11-20. doi: 10.1016/j.bcp.2018.03.011. Epub 2018 Mar 14.

引用本文的文献

1
Development of a Simple Protocol to Assess Glucose Release After In Vitro Digestion, Allowing Comparison of Starchy Foods.开发一种简单的体外消化后葡萄糖释放评估方案,用于比较淀粉类食物。
Food Sci Nutr. 2025 May 28;13(6):e70323. doi: 10.1002/fsn3.70323. eCollection 2025 Jun.
2
Gut microbes, diet, and genetics as drivers of metabolic liver disease: a narrative review outlining implications for precision medicine.肠道微生物、饮食和遗传作为代谢性肝病的驱动因素:一篇综述性叙述,概述了对精准医学的影响。
J Nutr Biochem. 2024 Nov;133:109704. doi: 10.1016/j.jnutbio.2024.109704. Epub 2024 Jul 17.
3
Effect of Sprouting on Biomolecular and Antioxidant Features of Common Buckwheat ().

本文引用的文献

1
Glutamate metabolism in a human intestinal epithelial cell layer model.人肠道上皮细胞层模型中的谷氨酸代谢。
Amino Acids. 2020 Dec;52(11-12):1505-1519. doi: 10.1007/s00726-020-02908-2. Epub 2020 Nov 12.
2
Glucose transporters in the small intestine in health and disease.肠道葡萄糖转运体在健康与疾病中的作用
Pflugers Arch. 2020 Sep;472(9):1207-1248. doi: 10.1007/s00424-020-02439-5. Epub 2020 Aug 23.
3
Cell-Type-Specific, Ketohexokinase-Dependent Induction by Fructose of Lipogenic Gene Expression in Mouse Small Intestine.
发芽对普通荞麦生物分子及抗氧化特性的影响()。 (注:括号部分原文缺失具体内容)
Foods. 2023 May 18;12(10):2047. doi: 10.3390/foods12102047.
4
Dietary Polyphenols and In Vitro Intestinal Fructose Uptake and Transport: A Systematic Literature Review.膳食多酚与体外肠道果糖摄取和转运:系统文献回顾。
Int J Mol Sci. 2022 Nov 18;23(22):14355. doi: 10.3390/ijms232214355.
果糖通过酮己糖激酶依赖性诱导小鼠小肠细胞特异性的脂基因表达。
J Nutr. 2020 Jul 1;150(7):1722-1730. doi: 10.1093/jn/nxaa113.
4
Olive oil by-product as functional ingredient in bakery products. Influence of processing and evaluation of biological effects.橄榄油副产物在烘焙产品中的功能性成分。加工的影响和生物效应的评估。
Food Res Int. 2020 May;131:108940. doi: 10.1016/j.foodres.2019.108940. Epub 2019 Dec 28.
5
Monocarboxylate Transporters (SLC16): Function, Regulation, and Role in Health and Disease.单羧酸转运蛋白(SLC16):功能、调节及其在健康和疾病中的作用。
Pharmacol Rev. 2020 Apr;72(2):466-485. doi: 10.1124/pr.119.018762.
6
Effects of Food Constituents on Absorption and Bioaccessibility of Dietary Synthetic Phenolic Antioxidant by Caco-2 Cells.食物成分对 Caco-2 细胞吸收和生物利用度的膳食合成酚类抗氧化剂的影响。
J Agric Food Chem. 2020 Apr 22;68(16):4670-4677. doi: 10.1021/acs.jafc.9b07315. Epub 2020 Mar 6.
7
Intestinal Fructose and Glucose Metabolism in Health and Disease.肠道果糖和葡萄糖代谢在健康和疾病中的作用。
Nutrients. 2019 Dec 29;12(1):94. doi: 10.3390/nu12010094.
8
A Dietary Intervention of Bioactive Enriched Foods Aimed at Adults at Risk of Metabolic Syndrome: Protocol and Results from PATHWAY-27 Pilot Study.富含生物活性物质的饮食干预对代谢综合征高危成年人的影响:PATHWAY-27 初步研究的方案和结果。
Nutrients. 2019 Aug 6;11(8):1814. doi: 10.3390/nu11081814.
9
Intestinal Saturated Long-Chain Fatty Acid, Glucose and Fructose Transporters and Their Inhibition by Natural Plant Extracts in Caco-2 Cells.Caco-2 细胞中肠道饱和长链脂肪酸、葡萄糖和果糖转运体及其天然植物提取物抑制作用。
Molecules. 2018 Oct 6;23(10):2544. doi: 10.3390/molecules23102544.
10
Olive oil industry by-products. Effects of a polyphenol-rich extract on the metabolome and response to inflammation in cultured intestinal cell.橄榄油产业副产物。多酚丰富提取物对培养肠细胞代谢组和炎症反应的影响。
Food Res Int. 2018 Nov;113:392-400. doi: 10.1016/j.foodres.2018.07.025. Epub 2018 Jul 17.