Meier N, Göringer H U, Kleuvers B, Scheibe U, Eberle J, Szymkowiak C, Zacharias M, Wagner R
FEBS Lett. 1986 Aug 11;204(1):89-95. doi: 10.1016/0014-5793(86)81392-8.
Methods of in vitro mutagenesis were employed to determine the importance of individual nucleotides within the ribosomal RNAs for the structure and function of E. coli ribosomes. A series of defined nucleotides in the genes for the 5 S and 16 S RNA were altered by transition and transversion mutations using either oligonucleotide-directed or bisulfite-catalyzed mutation procedures. Plasmids harbouring the mutated rRNA genes were expressed and the ribosomes containing such altered RNAs were investigated for impairments in RNA-protein interaction assembly and mRNA-coded tRNA binding.
