大肠杆菌16S rRNA倒数第二个茎中的碱基配对在核糖体亚基缔合中的重要性。
The importance of base pairing in the penultimate stem of Escherichia coli 16S rRNA for ribosomal subunit association.
作者信息
Firpo M A, Dahlberg A E
机构信息
Department of Molecular and Cell Biology and Biochemistry, Box G, Brown University, Providence, RI 02912, USA.
出版信息
Nucleic Acids Res. 1998 May 1;26(9):2156-60. doi: 10.1093/nar/26.9.2156.
Abstract
The influence of base pairing in the penultimate stem of Escherichia coli 16S rRNA (defined as nt 1409-1491) on ribosome function has been addressed by the construction of mutations in this region of rRNA. Two sets of mutations were made on either side of a structurally conserved region in the penultimate stem that disrupted base pairing, while a third set of mutations replaced the wild-type sequence with other base pair combinations. The effects of these mutations were analyzed in vivo and in vitro . The mutations that disrupted base pairing caused significant increases in cell doubling times as well as a severe subunit association defect and a modest increase in frame shifting and stop codon read-through. Restoration of base pairing restored wild-type growth rates, decoding and subunit association, indicating that base pairing in this region is essential for proper ribosome function.
摘要
通过构建大肠杆菌16S rRNA倒数第二个茎区（定义为核苷酸1409 - 1491）的突变，研究了该区域碱基配对对核糖体功能的影响。在倒数第二个茎区结构保守区域的两侧进行了两组突变，这些突变破坏了碱基配对，而第三组突变则用其他碱基对组合取代了野生型序列。在体内和体外分析了这些突变的影响。破坏碱基配对的突变导致细胞倍增时间显著增加，以及严重的亚基结合缺陷，同时移码和终止密码子通读略有增加。碱基配对的恢复恢复了野生型生长速率、解码和亚基结合，表明该区域的碱基配对对于核糖体的正常功能至关重要。
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