中国广州6至9岁儿童肠道微生物群组成和粪便短链脂肪酸与白细胞端粒长度的关联：一项横断面研究
Associations of the Gut Microbiota Composition and Fecal Short-Chain Fatty Acids with Leukocyte Telomere Length in Children Aged 6 to 9 Years in Guangzhou, China: A Cross-sectional Study.
作者信息
Chen Shan-Shan, Liao Xue-Mei, Wei Qin-Zhi, Zhou Ying-Yu, Su Meng-Yang, Hu Yan, Song Yan-Yan, Zhang Zhe-Qing, Liang Jing-Jing
机构信息
Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
Food Safety and Health Research Center, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
出版信息
J Nutr. 2022 Jun 9;152(6):1549-1559. doi: 10.1093/jn/nxac063.
BACKGROUND
Telomere length (TL) serves as a marker of cellular senescence and appears to plateau between the age of 4 y and young adulthood, after which the gut microbiota are supposed to be established. However, scarce data are available regarding the correlation between gut microbiota composition and TL in the pediatric population.
OBJECTIVES
We aimed to investigate whether the gut microbiota and the concentrations of SCFAs in feces are associated with leukocyte TL in children.
METHODS
In total, 401 children aged 6-9 y from Guangzhou were enrolled in this cross-sectional study. qPCR was used to determine relative TL in peripheral blood leukocytes. The gut microbiota was characterized by 16S ribosomal RNA amplicon sequencing and the fecal concentrations of total SCFAs and SCFA subtypes were determined using HPLC. The multivariate methods with an unbiased variable selection (MUVR) algorithm and partial least square models were used to select predictable operational taxonomic units (OTUs). Further correlation analyses were performed based on multiple linear regression models with adjustment for covariates and false discovery rate.
RESULTS
With the use of MUVR, 35 relevant and minimal optimal OTUs were finally selected. Multiple linear regression analysis showed that the abundance of several OTUs, including OTU334 (belonging to the genus Family XIII AD3011 group), OTU726 (belonging to the species Lachnoclostridium phocaeense), OTU1441 (belonging to the genus Ruminococcus torques group), OTU2553 (belonging to the genus Lachnospiraceae UCG-010), and OTU3375 (belonging to the family Lachnospiraceae), was negatively associated with leukocyte TL (β: -0.187 to -0.142; false discovery rate (FDR)-corrected P value (PFDR) = 0.009-0.035]. However, neither SCFA subtype nor total SCFA content in feces exhibited significant associations with TL (β: -0.032 to 0.048; PFDR = 0.915-0.969).
CONCLUSIONS
The gut microbiota, but not fecal SCFA concentration, was significantly associated with TL in this pediatric population.
背景
端粒长度（TL）是细胞衰老的标志物，在4岁至青年期之间似乎趋于稳定，此后肠道微生物群应该已经建立。然而，关于儿科人群中肠道微生物群组成与端粒长度之间的相关性，现有数据稀缺。
目的
我们旨在研究儿童的肠道微生物群和粪便中短链脂肪酸（SCFA）的浓度是否与白细胞端粒长度相关。
方法
本横断面研究共纳入了401名来自广州的6至9岁儿童。采用定量聚合酶链反应（qPCR）测定外周血白细胞中的相对端粒长度。通过16S核糖体RNA扩增子测序对肠道微生物群进行表征，并使用高效液相色谱法测定粪便中总短链脂肪酸和短链脂肪酸亚型的浓度。使用具有无偏变量选择（MUVR）算法的多变量方法和偏最小二乘模型来选择可预测的操作分类单元（OTU）。基于多元线性回归模型进行进一步的相关性分析，并对协变量和错误发现率进行调整。
结果
使用MUVR最终选择了35个相关且最小最优的OTU。多元线性回归分析表明，包括OTU334（属于XIII AD3011科属）、OTU726（属于菲氏瘤胃球菌种）、OTU1441（属于扭链瘤胃球菌属组）、OTU2553（属于毛螺菌科UCG-010属）和OTU3375（属于毛螺菌科）在内的几个OTU的丰度与白细胞端粒长度呈负相关（β：-0.187至-0.142；错误发现率（FDR）校正P值（PFDR）=0.009至0.035）。然而，粪便中的短链脂肪酸亚型和总短链脂肪酸含量均未显示与端粒长度有显著相关性（β：-0.032至0.048；PFDR=0.915至0.969）。
结论
在这个儿科人群中，肠道微生物群与端粒长度显著相关，而粪便中短链脂肪酸浓度与端粒长度无关。
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