Zhang Wenfei, Gu Xingwang, Zhao Qing, Wang Chuting, Liu Xinyu, Chen Youxin, Zhao Xinyu
Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Key Laboratory of Ocular Fundus Diseases, Chinese Academy of Medical Sciences, Beijing, China.
Front Med (Lausanne). 2024 Jun 4;11:1411271. doi: 10.3389/fmed.2024.1411271. eCollection 2024.
To investigate the causal relationship between gut microbiota (GM) and chalazion through Mendelian randomization (MR) analysis.
GM-related genome-wide association studies (GWAS) were obtained from the International Consortium MiBioGen. Genetic data for chalazion were sourced from the MRC Integrative Epidemiology Unit (IEU) Open GWAS database. Five MR methods, including inverse variance weighted (IVW), were employed to estimate causal relationships. Cochran's Q test was used to detect heterogeneity, the MR-Egger intercept test and MR-PRESSO regression were utilized to detect horizontal pleiotropy, and the leave-one-out method was employed to validate data stability.
We identified 1,509 single nucleotide polymorphisms (SNPs) across 119 genera as instrumental variables (IVs) ( < 1 × 10). According to the inverse variance weighted (IVW) estimate, the Family XIII AD3011 group (OR = 1.0018, 95% CI 1.0002-1.0035, = 0.030) and Catenibacterium (OR = 1.0013, 95% CI 1.0002-1.0025, = 0.022) were potentially associated with increased risk of chalazion. Conversely, Veillonella (OR = 0.9986, 95% CI 0.9974-0.9999, = 0.036) appeared to provide protection against chalazion. There was no evidence of heterogeneity or pleiotropy.
This study uncovered the causal relationship between GM and chalazion, pinpointing Catenibacterium and Family XIII AD3011 group as potential risk contributors, while highlighting Veillonella as a protective factor. In-depth investigation into the potential mechanisms of specific bacteria in chalazion was essential for providing novel therapeutic and preventive strategies in the future.
通过孟德尔随机化（MR）分析研究肠道微生物群（GM）与睑板腺囊肿之间的因果关系。
GM相关的全基因组关联研究（GWAS）数据来自国际微生物组联盟MiBioGen。睑板腺囊肿的遗传数据来自医学研究理事会综合流行病学单位（MRC IEU）开放GWAS数据库。采用包括逆方差加权（IVW）在内的五种MR方法来估计因果关系。使用 Cochr an's Q检验检测异质性，采用MR-Egger截距检验和MR-PRESSO回归检测水平多效性，并采用留一法验证数据稳定性。
我们确定了119个属中的1509个单核苷酸多态性（SNP）作为工具变量（IVs）（<1×10）。根据逆方差加权（IVW）估计，第十三族AD3011组（OR = 1.0018，95%CI 1.0002 - 1.0035，P = 0.030）和链状杆菌属（OR = 1.0013，95%CI 1.0002 - 1.0025，P = 0.022）可能与睑板腺囊肿风险增加有关。相反，韦荣球菌属（OR = 0.9986，95%CI 0.9974 - 0.9999，P = 0.036）似乎对睑板腺囊肿有保护作用。没有异质性或多效性的证据。
本研究揭示了GM与睑板腺囊肿之间的因果关系，确定链状杆菌属和第十三族AD3011组为潜在风险因素，同时突出韦荣球菌属作为保护因素。深入研究特定细菌在睑板腺囊肿中的潜在机制对于未来提供新的治疗和预防策略至关重要。
