School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China.
Unconventional gas research institute, Henan Polytechnic University, Jiaozuo 454000, China; Collaborative Innovation Center of Coalbed Methane and Shale Gas for Central Plains Economic Region, Jiaozuo 454000, China; School of Energy Resources, China University of Geosciences, Wuhan 430074, China.
J Biotechnol. 2022 Mar 20;348:26-35. doi: 10.1016/j.jbiotec.2022.03.002. Epub 2022 Mar 9.
The culture medium in biogas field have been used in coalbed gas bioengineering (CBGB). However, there is a huge difference between the substrate of biogas fermentation and coal. It is necessary to study and optimize the culture medium in the anaerobic digestion (AD) system with coal as substrate. In this study, the single factor test and response surface curve analysis are used to clarify the essential components in the culture medium and the optimal content of these chemicals. The influence of a single component on microbial community structure and major metabolic pathways in AD system are discussed. Under the optimal conditions, SEM observation show that the coal surface sediment is significantly reduced after AD process. The results of GC-MS show that there is no significant difference in the composition and content of organic compounds in the liquid phase before and after the optimization; the microbial community structure and gene function did not weaken with the decrease of culture medium addition, but formed a more targeted and stable microbial community.
沼气领域的培养基已被用于煤层气生物工程（CBGB）。然而，沼气发酵和煤的基质之间存在巨大差异。有必要研究和优化以煤为基质的厌氧消化（AD）系统中的培养基。在本研究中，采用单因素试验和响应面曲线分析来阐明培养基中的基本成分以及这些化学物质的最佳含量。讨论了单一成分对 AD 系统中微生物群落结构和主要代谢途径的影响。在最佳条件下，SEM 观察表明 AD 过程后煤表面沉积物明显减少。GC-MS 的结果表明，优化前后液相中有机化合物的组成和含量没有明显差异；随着培养基添加量的减少，微生物群落结构和基因功能并没有减弱，而是形成了一个更有针对性和稳定的微生物群落。
